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Sulfated cyclodextrins with more than one- half of the possible sites sulfated are referred to as “highly sulfated.” Highly sulfated cyclodextrins with average degrees
of sulfation of 11, 12, 13 for α-, β-, γ-CD, respectively, with a narrow range of heterogeneity and low batch-to-batch variations, were synthesized and used in CE by Evangelista and Chen (13). These reagents are manufactured by the supplier using a controlled sulfation reaction and formulated to produce consistent lots. All HSCDs are developed using strict quality control procedures which test the reagents’ consistency in functional assays.
Finding a generic method for chiral separations has been a challenge for many years. A unique group of drugs and
metabolites, expected to be found after administration to the general population, was used to evaluate the highly sulfated cyclodextrin strategy. These 101 racemic drugs and metabolites are of interest to both the pharmaceutical and forensic communities. The compounds were screened and baseline resolution was achieved for over 94% of the group without method optimization. The result confirms the usefulness of this rapid solution to complex enantiomeric separations.
References 1. Chapman, J.D. and A.-F. Chen, LCGC Europe, January 33 - 37 (2001)
2. Chen, F -T.A. Shen, G and R.A. Evangelista, J. Chromatogr. A 924 (2001) 523.
3. Hudson, J.C. et al., Can.
Soc.Forens. Sci., 31(1) 1 - 29 (1998)
4. Issaq, H. J. J. Liq. Chromatogr. Relat. Technol. 25, (2002), 1153.
5. R. Vespalec and P. Bocek, Chem Rev. 100, (2000), 3715
6. K . Verleysen and P. Sandra, Electrophoresis 19 (1998) 2798.
7. B . Chankvetadze, J. Chromatogr. A 792 (1997) 269.
8. B . Chankvetadze and G. Blaschke, J. Chromatogr. A 906 (2001)
9. A . Amini, Electrophoresis 22 (2001) 3107.
10. E .C. Rickard and R.J. Bopp, J. Chromatogr. 680 (1994) 609.
11. I .E. Valko, H.A.H. Billiet, J. Frank and K.C.A.M. Luyben, J. Chromatogr. 678 (1994) 139.
12. L . Liu, L.M. Osborne andM.A. Nussbaum, J. Chromatogr. A 745 (1996) 45.
13. Chen, F.T.A. and R.A. Evangelista, J. Chin. Chem. Soc. 46, (1999), 847.
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