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Microscopy 101


simple, quick, and inexpensive method for producing a greater range of orientations of diatom frustules and valves compared with using a flat mount.


Acknowledgements Our thanks to Rafael Hoekstra and Arjan van Asselt for lab


assistance, Liz Girvan for SEM technical support at the University of Otago, New Zealand, and support from the Advanced Microscopy Facility at the University of Victoria, Canada.


References [1] M Hildebrand, “Marine Diatoms and Teir Role in the Ecosystem,”


https://aaas.confex.com/aaas/2010/webpro- gram/Paper1743.html.


[2] ML MacGillivary and JM Ehrman, Diatom Research 26(4) (2011) 321–23.


[3] FE Round, Annals of Botany 53 (1984) 457–68. [4] F Round et al., Te Diatoms, Biology & Morphology of the Genera, Cambridge University Press, Cambridge, UK, 1990.


[5] GR Hasle and EE Syvertsen, “Marine Diatoms” in Iden- tifying Marine Phytoplankton, ed. CR Tomas, Academic Press, San Diego, 1997, pp. 334–36.


[6] J Pickett-Heaps, J Phycol 34 (1998) 1088–89. [7] I Jameson and GM Hallegraeff, Algae of Australia. Phyto- plankton of temperate waters, eds. GM Hallegraeff et al., Canberra & Melbourne: ABRS; CSIRO Publishing, Clay- ton, Australia, 2010, pp. 16–82.


[8] SS Lee et al., Diatom Research 29(4) (2014) 325–50. 


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