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three assay kits differ according to the range of concentrations they can measure (broad range, high sensitivity and ultra high sensitivity). When combined with the DS-11 FX, the Ultra High Sensitivity kit allows quantification down to 0.5 pg/ µL dsDNA. Te combination of absorbance and fluorescence measurements gives the instrument an overall dynamic range from 0.5 pg/µL to 37,500 ng/µL. One recent application
involved extracting DNA from blood in the stomach of mites. Te customer had developed methods for amplifying the DNA but contamination of the samples with other nucleic acids and the very low concentrations meant that absorbance alone was not enough to get reliable quantification. By using a combination of the fluorescence Ultra High Sensitivity kit and taking absorbance measurements the customer was able to get a specific quantification while also gaining a good picture of the contaminants present in their samples.
Real-time analysis Hiden’s new HPR-40 DSA membrane inlet mass spectrometer (MIMS) is a bench-top gas analysis system for real-time quantitative analysis and monitoring of dissolved/evolved gases. Te system offers the facility for analysis to sub-ppb (parts per billion) levels and is suited to gas analysis applications where sample volume is small and for environmental applications where detection of a low concentration level is required, providing analysis of dissolved species with a mass range of 200 amu, with a 300 amu option. To support a wide range of applications, both dissolved species probe inlets and circular membrane carrier inlets are available. Te inlet probe uses a special membrane that allows small levels of the dissolved
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species to pass through it and into the ion source of a high precision quadrupole mass spectrometer. A manual isolation valve allows for control of the sampling and a solenoid safety valve provides protection for the mass spectrometer and vacuum system in case of membrane failure. Hiden predicts the HPR-40 will be a measurement asset in areas such as fermentation processing, photosynthesis, electrochemical reaction studies, water quality and soil condition assessment, plant root performance and analysis of enzyme kinetics.
Industry standard US company International Light Technologies describes its newest generation of product as “the ILT1700 for the 21st century”, with improvements
on the original device including rapid optical measurements (1-100Hz), a broader dynamic range (100fA to 1mA), extensive software apps, data storage and rechargeable batteries. Te ILT5000 can be operated wirelessly and comes with a basic Labview
The ILT5000 is backward compatible
The HPR-40 DSA bench-top MIMS from Hiden
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