34 CHROMATOGRAPHY


lab developed tests have become increasingly popular because the technology offers the flexibility to develop cutting edge in-house tests to measure clinically relevant compounds, as well as metabolites, and small volumes of samples with confidence. Te coupling of HPLC and MS, can offer in the space of a few minutes separation, identification and quantification of hundreds of analytes in a complex biological matrix. Clinical laboratories are gradually increasing the volume of lab-developed tests traditionally done on HPLC with UV detection or GCMS technology because it’s generally faster and cheaper in the long run.


“In the future, leaders in the clinical laboratories will continue to see more analysis


moving to HPLC MS- based workflows moving toward larger molecule analysis of peptides and proteins,” says Tomas. “Most promising clinical research areas continue to be in endocrinology, inborn errors of metabolism, and therapeutic drug monitoring research. With the focus on precision medicine, medical scientists continue to ensure they can better understand how to administer the right treatment at the right time for the right person in the right amount; particularly for disease areas such as cancer.” For complex biological samples, Termo Scientific uses its TurboFlow, which Tomas says improves MS detector response to analytes of interest (for instance, testosterone) by removal of non-analyte


Professor Chris Probert from the University of Liverpool


MALDI-TOF is an imaging technique that allows identification and structural characterisation of biomolecules


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