38 CHROMATOGRAPHY
ACCURACY in AIR TESTING
Shun-Hsin Liang explains how to improve analysis of aldehydes and ketones in air samples with faster, more accurate methodology
A
ccurate analysis of aldehydes and ketones in air samples is an
important part of monitoring programmes that are used to control air pollution and to protect human health around the world. Tese airborne carbonyls from industrial and vehicle emissions are precursors to ozone in smog and are associated with respiratory and pulmonary problems. In addition, specific carbonyls – formaldehyde and acetaldehyde – have been identified as known or suspected carcinogens. Prior to analysis, aldehydes and ketones are converted to hydrazone derivatives when they are captured on a sampling tube composed of silica gel coated with 2,4-dinitrophenylhydrazine (DNPH). Ten, the aldehyde/ ketone DNPH derivatives are eluted from the sampling tube with solvent and analysed by HPLC-UV. Typically, carbonyl LC methods require long analysis times (often over an hour), but many still fail to provide adequate resolution for some aldehyde/ketone DNPH derivatives.
To help labs improve analytical speed and accuracy in the analysis of aldehydes and ketones in air samples, Restek has developed LC columns, instrument conditions and certified reference materials that reliably provide baseline separation of commonly analysed aldehyde/ketone DNPH derivatives in just minutes. For example, airborne carbonyls LC method US EPA Method TO-11A uses a conventional C18 column and requires an analysis time of over one hour, yet acrolein and acetone partially coelute and the o-, m-, and p- tolualdehyde isomers cannot be fully separated. In contrast, using a Raptor C18 column under the conditions shown below all 15 carbonyls – including the tolualdehyde isomer cluster – are separated to baseline in a fast 14-minute analysis. Similarly, airborne carbonyl
LC method CARB 1004 produces extremely long analysis times; in fact, this method requires two separate chromatographic analyses to quantify all compounds. Te first is a 37-minute analysis
Baseline separation ensures accurate determination of 15 commonly analysed aldehydes and ketones in air samples
All 15 carbonyls – including the tolualdehyde isomer cluster – are separated to baseline in a fast 14-minute analysis
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