44 CHROMATOGRAPHY
Waters’ new in vitro diagnostic system is designed to tackle the most demanding of quantitative analyses.
this elite LC system provides high resolution, sensitivity and improved throughput with exceptional, reliable results, adds the company.
Preparation time One of the key issues tackled with Altus is preparation time. Tis is reduced significantly with AutoBlend technology, which automates the formulation of mobile phases from reservoirs of pure solvents or concentrated stock solutions. AutoBlend Plus also manages pH and ionic strength requirements for the selected mobile phase automatically, so users can take
full advantage of the system’s quaternary blending capabilities.
Te company cites a number of additional benefits, including multi-solvent blending, direct inject sampling, next-generation column compartments, managed dwell volume, and a full complement of detection modes. Also, it is easy to transfer methods from current instruments to the Altus UPLC system. Users simply select a column comparable to their HPLC column, enter existing HPLC conditions into the system’s UPLC Columns Calculator, and run.
What limits your HPLC system? W
hen was the last time that you couldn’t sufficiently separate some peaks of interest? How did this affect your analysis?
If you own a standard HPLC system and normally
perform separations on 5 µm or 3 µm columns, you have a great chance for improving this lack of separating power. However, according to Knauer, there are some issues to take into account before investing time and money in new methods or equipment. First is the fitness of the LC system. For example, if you do not own a system with low dead volume,
www.scientistlive.com
capable of delivering solvent at more than 600 bar, there will only be a few applications on fully porous sub-2 µm columns that are suitable for your HPLC. According to Knauer, core-shell or superficially
porous columns might be worth a try, because of their lower back pressure. But you need to be sure that system dead volume and your detector flow cell don’t spoil the improved separation results of the column. If the gain in resolution with the new column is
high enough you could even speed up your analysis by using shorter columns.
Then there is scalabililty. For example, if you use
your system for screening and the methods you develop are intended for being easily transferred to a preparative scale HPLC, fully porous particle columns are your best choice, adds the company. For a solution that is capable of mastering both of these situations and offering laboratories a much greater freedom of choice, Knauer says that its PLATINblue UHPLC/HPLC Plus system is ideal. The company’s website (
www.knauer.net) has more information on both this and its other solutions.
For Shimadzu, high performance across a wider application range and improving flexibility and reliability of HPLC/UHPLC analyses have been the driving forces behind development of its Nexera X2 series.
Like its predecessor, the Nexera X2 is optimised to enable analysis at pressures up to 19,000 psi without compromising performance and data quality. Nexera X2 autosamplers provide near-zero carryover, support ultrafast analysis with what the company says is the world’s fastest cycle time (14 seconds), and deliver excellent injection
reproducibility down to 0.1µL injection volumes. In addition, the Nexera X2 systems can be configured with a variety of solvent delivery units and column ovens to meet any laboratory workflow.
Shimadzu highlights an extra benefit, which is the system’s modular construction. Tis allows a solvent blending function that can mix up to four solvents per pump at a desired ratio, letting users change the proportion of the mobile phase components from run to run so there is no solvent wasted.
Page 1 |
Page 2 |
Page 3 |
Page 4 |
Page 5 |
Page 6 |
Page 7 |
Page 8 |
Page 9 |
Page 10 |
Page 11 |
Page 12 |
Page 13 |
Page 14 |
Page 15 |
Page 16 |
Page 17 |
Page 18 |
Page 19 |
Page 20 |
Page 21 |
Page 22 |
Page 23 |
Page 24 |
Page 25 |
Page 26 |
Page 27 |
Page 28 |
Page 29 |
Page 30 |
Page 31 |
Page 32 |
Page 33 |
Page 34 |
Page 35 |
Page 36 |
Page 37 |
Page 38 |
Page 39 |
Page 40 |
Page 41 |
Page 42 |
Page 43 |
Page 44 |
Page 45 |
Page 46 |
Page 47 |
Page 48 |
Page 49 |
Page 50 |
Page 51 |
Page 52 |
Page 53 |
Page 54 |
Page 55 |
Page 56 |
Page 57 |
Page 58 |
Page 59 |
Page 60 |
Page 61 |
Page 62 |
Page 63 |
Page 64 |
Page 65 |
Page 66 |
Page 67 |
Page 68 |
Page 69 |
Page 70 |
Page 71 |
Page 72 |
Page 73 |
Page 74 |
Page 75 |
Page 76 |
Page 77 |
Page 78 |
Page 79 |
Page 80 |
Page 81 |
Page 82 |
Page 83 |
Page 84 |
Page 85 |
Page 86 |
Page 87 |
Page 88 |
Page 89 |
Page 90 |
Page 91 |
Page 92