CHROMATOGRAPHY 43
Advanced LC techniques focus on ultrafast analysis
Ongoing developments in ultra performance liquid chromatography and ultra high performance liquid chromatography technology is making seamless integration into existing laboratory systems much simpler, says Eugene McCarthy.
Les développements en cours en chromatographie liquide haute performance ultra et la technologie de chromatographie liquide à haute performance ultra est de faire une intégration transparente dans les systèmes de laboratoire existantes beaucoup plus simples, dit Eugene McCarthy.
Weiterentwicklungen in extrem Flüssigkeits-Chromatographie und Ultrahochleistungsflüssigkeits -Chromatographie -Technologie ist die nahtlose Integration in bestehende Laborsystemen viel einfacher , sagt Eugene McCarthy.
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off-axis ion source technology, and radar, an information-rich acquisition method, enabling the system with sensitivity and robustness.
One early adopter of the new system is the Chinese Food and Drug Administration (CFDA) which has approved it for IVD use in the country. So it can now be used in China for the clinical analysis of a variety of compounds including diagnostic indicators and compounds for treatment monitoring.
linical laboratories today typically use LC-MS technology to perform
qualitative and quantitative analyses of patients’ samples. Such tests cover a variety of uses, for example: to confirm a clinical suspicion (including making a diagnosis); assist in the selection, optimisation and monitoring of treatment; provide a prognosis; screen for disease in the absence of clinical signs or symptoms; and establish and monitor the severity of physiological disturbance.
Liquid chromatography separates analytes and interferents within a given sample, while mass spectrometry technology is used for detection and confirmation of those analytes.
Te new Waters Acquity UPLC I-Class IVD/Xevo TQ-S in vitro diagnostic (IVD) system features the company’s UltraPerformance LC (UPLC) technology coupled with a tandem quadrupole mass spectrometer designed for the most demanding UPLC-MS/MS quantitative analysis. Tis system uses StepWave, which Waters describes as a breakthrough
According to Jeff Mazzeo, senior director, health sciences for Waters Division, improved diagnostic and predictive tools such as this are essential to help medicine move from a reactive approach to treating disease to one that is more proactive and preventive.
In related news, Waters Acquity UPLC received an award at the recent Pittcon conference on analytical chemistry and applied spectroscopy as a result of good customer reviews and ratings. Te technology allows chromatographers to work at higher efficiencies with a much wider range of linear velocities, flow rates, and backpressures. Te company says that what differentiates it from other UPLC systems is Waters’ patented 1.7 micron hybrid particle chemistry.
Agilent technologies is also expanding its UHPLC portfolio with the 1290 Infinity II LC system – the latest addition to its 1200 Infinity series LC portfolio.
Te company has particularly focused on analytical quality, ease of use and seamless
integration with its new system as it strives to help laboratory scientists reach new levels of efficiency. In development, Agilent worked with a variety of customers in order to identify their most challenging liquid chromatography workflow issues and bottlenecks.
Tis involved three main priority areas for development. First is analytical efficiency. Here it has combined chromatographic resolution/precision and broad dynamic range detection with low carry-over.
Te second priority is instrument efficiency. Maximum flexibility to accommodate changing separation conditions reduces turnaround times, while fast throughput is enabled by high sample capacity per bench space and fastest injection cycles.
Lastly is laboratory efficiency, and this is enabled by allowing the smooth transfer from any legacy equipment – always an important factor when laboratories are seeking to lower costs and increase their return on investment.
Te analysis of complex samples is the target of the new Altus UPLC from PerkinElmer. Te company says its new technology represents a ‘breakthrough’ in LC particle technology and system design, combining as it does advanced fluidics with sub-2µm particle columns for what PerkinElmer describes as superb performance at high pressures, with minimal volumes and optimised flow paths.
Combining the flexibility of quaternary solvent blending with the flow-through needle injector,
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