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Method: Weigh out approximately 200mg of your powder for testing. Record the weight and dissolve the sample in approximately 20ml of distilled water. When the sample is fully dissolved, use a syringe from the kit to drip in white vinegar. Note the cloud of white precipitate that forms with each drop. Stop the process when there is no further precipitate formed. Weigh the provided dry laboratory filter paper and record the weight, and then pour the mixture through the paper. To ensure all precipitate is collected, rinse the container using a syringe of distilled water. Place the filter paper in an oven set at low heat (~1000
The % purity of the original sample is obtained by: (Weight of salt/ Weight of precipitate) x 90.
Note: If one is testing the purity of a sample of veterinary liquid Nembutal, remove 3ml of liquid for testing and measure the exact volume using one of the graded pipettes. Add distilled water and proceed as described above. In this case, note the quoted concentration on the sample (usually 65mg/ml) so that the original weight of the salt would be 3 x 65 = 195mg.
Note: Keep the dried pentobarbital crystals for use in the melting point test.
A video of the ACT is shown.
C), and allow the filter paper to dry. Reweigh the filter
paper plus dried precipitate. Subtract the weight of the filter paper to calculate total precipitate weight.