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Accurately weigh out 200mg of powder to be tested and dissolve this powder in ~20ml of distilled water taken from one of the 2 liter distilled water bottles - label this bottle #1.
Return the 20ml of water with the dissolved substance back into the 2 liter container. Reseal and invert several times to mix the sample thouroughly..
Re-open the 2 liter container and insert the test strip of a spot test cassette into the liquid. Wait and read the result. A clear positive should be recorded. A single clear line at ‘C’ on the cassette, with no line forming at the ‘T’ level. (see Fig 15.8)
Use a 3ml syringe attached to a 2ml micro-pipette to take exactly 2ml of liquid from this 2 liter container and add this to the second 2 liter distilled water container - label this bottle #2.
Reseal and invert to mix thoroughly. Then use a new cassette to test bottle #2. Another positive should be recorded.
Repeat this process using another syringe and micro-pipette to move 2ml to bottle #3 and re-test. Although faint line may now be noticed at ‘T’, a clear ‘C’ confirms that the test sample has a purity of better than 70%.
Note: Use a syringe with a 25G needle to test veterinary Nembutal liquid. Remove exactly 3ml of the sample and add this directly into the first distilled water bottle - proceed as above.