AURION IMMUNOGOLD REAGENTS EDITION VI PRODUCT INFORMATION


zzz Ultra Small ImmunoGold Reagents


Aurion Ultra Small Immunogold Reagents are prepared with subnanometer gold particles. These particles have far less influence on the adsorbed antibodies or detecting molecules, and consequently the conjugates behave as though they are uncoupled. In conjunction with the highly efficient and easy-to-use R-Gent SE-Intense and SE-EM silver enhancement reagents, the Ultra Small Immunogold Reagents are the best choice for any application.


Introduction


Reduction of the gold particle size provides Ultra Small Immunogold Reagents with fundamentally dif- ferent characteristics when compared with conjugates built around larger particles. While the Conventional Immunogold Reagents can be thought of as particles coated with proteins, Ultra Small Immunogold Reagents are proteins coated with one or more gold particles. With this structure, both the overall size of the conjugates, as well as steric hindrance are decreased.


TECHNICAL TIPS...


Application Instructions Electron Microscopy Labeling


For post-embedding labeling, incubate thin sections by placing grids on drops of reagent arrayed on a sheet of Parafilm™. The use of nickel grids is recommended, especially if silver enhancement procedures are intended.


For pre-embedding labeling, float specimens in dilute reagent on a rocking table.


For more information on post and pre- embedding labeling; • Post-embedding Immuno Incubation Protocol


• Pre-embedding Immuno Incubation Protocol • Ultra Small Gold Reagents and R-Gent SE-EM in Pre-embedding single and double labeling


Light Microscopy Slide or Coverslip Labeling


Apply a few drops of dilute reagent to cover the specimen.


Recommended Incubation Solution: PBS,


(10 mM Phosphate buffer, 150 mM NaCl) 0.1-0.2 % AURION BSA-c™ 15 mM NaN3 pH 7.4


Additional Information


“Ultra Small Gold Reagents and R-Gent SE-EM in Pre-embedding Single and Double Labeling” Hong Yi and Jan L.M. Leunissen


“Optimized Immuno Labeling using AURION BSA-c™ and AURION Blocking Solutions” by J.L.M. Leunissen and P.F.E.M. van de Plas


For additional information, please see the Aurion Immunogold Newsletters and Technical Tips on our website.


4 Particle


Diameter 0.8 nm 6.0 nm 10 nm


+⁄– #Au atoms 15


6500 0·103


+⁄– MWt. (daltons) 3·103


1.3·106 6·106


Aurion offers Ultra Small Immunogold Reagents with an average gold particle diameter of 0.8 nm or less. These ultra small gold particles can be visualized directly in high angle annular dark-field-scanning TEM. However, the gold signal is normally visualized after increasing the particle diameter with silver enhancement. The reagents can be used in elec- tron and light microscopy as well as in blotting experiments. The universal applicability makes it easy to compare results obtained with different procedures.


Product Description


AURION Ultra Small Immunogold Reagents contain 60-80 µg of specific protein/ml for IgG conjugates. F(ab')2, Fab and biotinylated albumin conjugates contain equimolar amounts of conjugated protein. The average gold cluster diameter is less than 0.8 nm.


+⁄– #Particles /ml


5·1015


2.4·1013 5·1012


+⁄– #Ab (/part) 0.1-1? 1-2


7-12


The small gold particles also have a tight particle surface curvature which makes it less likely that a structured water dipole layer will build-up around the gold parti- cles. Hence, the hydrodynamic radius of the ultra small gold colloids is reduced. Finally, small gold particles carry less net negative charge; thus, they undergo less charge determined repulsion when approaching the sample surface.


AURION Ultra Small Immunogold Reagents are used in conjunction with AURION R-Gent SE-EM or SE-Intense silver enhancement reagents, developed for electron microscopy and light microscopy/ immunoblotting respectively.


The reagents are supplied in PBS with 1% Bovine Serum Albumin and 15 mM NaN3.


The activity of each lot is determined using a dot-spot test system as described by Moeremans et al., J. Immunol. Methods, 74, (1984), 353.


The products are available in two package sizes: Regular package: for 1200 grids or 600 slides, Small package: for 480 grids or 240 slides. Specificity


Aurion offers the widest range of Ultra Small Immunogold Reagents. The most commonly used reagent types are available as intact IgG, F(ab')2 and Single Fab conjugates. To view the complete list of avail- able reagents click here.


AURION Ultra Small Immunogold Reagents are prepared using the highest quality antibodies or binding agents available. All antibodies are immuno affinity purified and immuno cross-adsorbed to reduce non- specific interactions.


Storage


AURION Immunogold Reagents have a guaranteed shelf life of 18 months from the date of quality con- trol analysis.


The products should be stored at 4-8°C. Freezing is not recommended. zzz Ultra-Small Gold Conjugate Starter Kit, see page 16 >>>


Page 1  |  Page 2  |  Page 3  |  Page 4  |  Page 5  |  Page 6  |  Page 7  |  Page 8  |  Page 9  |  Page 10  |  Page 11  |  Page 12  |  Page 13  |  Page 14  |  Page 15  |  Page 16  |  Page 17  |  Page 18  |  Page 19  |  Page 20  |  Page 21  |  Page 22  |  Page 23  |  Page 24  |  Page 25  |  Page 26  |  Page 27  |  Page 28