AURION IMMUNOGOLD REAGENTS EDITION VI PRODUCT INFORMATION


zzz Gold Nanoparticles – Gold Sols


To prepare a high quality (immuno)gold conjugate it is important to have particles with uniform size and highly adsorptive surfaces. The Aurion gold sols are prepared according to unique production protocols. This provides for sol particles with the same narrow size distribution and adsorption properties as employed in the Conventional Immunogold Reagents.


Introduction


The preparation of conventional gold reagents is based on gold particles with a diameter suited for direct electron microscopic visualization. Aurion offers a range of Conventional Immunogold Reagents which cover the majority of approaches in transmission and scanning electron microscopy.


Aurion Gold Sols (solutions of high quality unconjugated gold particles) provide opportunities for users to prepare conjugates with primary antibodies, ligands and other binding agents with the same particle characteristics as in the Conventional Immunogold Reagents.


Product Description


AURION Gold Sols are prepared according to a unique protocol, warranting narrow size distribution and reproducible adsorption characteristics.


The AURION gold sols are available in the same size range as the Conventional Immunogold Reagents: 6, 10, 15 and 25 nm. The particle population is monodisperse and thus shows minimal size variation and overlap. Typically, the coefficient of variance for the 6 and 25 nm particle size sols is less than 12%, whereas the 10 and 15 nm size sols show less then 10% variation. Actual lot specifications (size, variation and expiry date) are indicated on the accompanying package insert.


Package size: 100 ml of high quality gold sol at an OD520nm of approximately 1.


Particle size and size distributions of the AURION Gold Nanoparticles – Gold Sols


TECHNICAL TIPS... Application Instructions


6 nm 10 nm 15 nm


TECHNICAL TIPS... Application Instructions


Detailed information is provided in the package inserts. Storage


AURION gold sols have a guaranteed shelf life of 12 months from the date of quality control analysis.


The products should be stored at 4-8°C. Freezing is not recommended. 25 nm


Detailed information is provided in the package insert. Col-Aurion is a sensitive total protein stain for bio assays, especially nitrocellulose and PVDF Western blots and dot-blots. The protein binding substratum has to carry a negative charge.


Storage


Col-Aurion has a guaranteed shelf life of 18 months from the date of quality control analysis. Store at 4-8°C. Do not freeze.


Additional Information


For additional information, please see the Aurion Immunogold Newsletters and Technical Tips on our website.


Aurion Diagnostics Gold Nanoparticles


Now available for the fields of Diagnostics and Diagnostics Development....


See Inside Front Cover for more information.


13 NEW!...


zzz Colloidal Gold Based Protein Stain


Col-Aurion is a colloidal gold particle based total protein stain, developed for the sensitive staining of


electrophoretically separated protein bands on nitrocellulose or PVDF™ blotting membranes. Colloidal gold particles accumulate at the site of the protein bands on the membrane, generating an intense dark red staining pattern. The total protein stain assists in assessing immunoblotting results and to evaluate the effectivity of the blotting procedure.


Introduction


The negative surface charge of colloidal gold particles is responsible for their high affinity for positively charged macromolecules. This characteristic was the basis for the development of a total protein stain based on colloidal gold.


Product Description


Col-Aurion is a total protein stain consisting of a solution of coated gold particles with an average particle diameter of 15 nm. The staining principle is based on the electrostatic binding of negatively charged gold particles to proteins with a positive charge present at low pH.


A unique feature of Col-Aurion is the use of BSA-c™ to shield off the surface of the gold particles. Destabilization of gold particles that might occur as a result of interaction with detached protein is thus prevented. The strong negative charge of BSA-c™ gives an additional increase in sensitivity of the stain.


Col-Aurion total protein stain has a pH of 3.2 and is ready to use. For removal of surplus of weakly bound protein from the membrane, a 10 ml vial of Tween-20™ is included.


Page 1  |  Page 2  |  Page 3  |  Page 4  |  Page 5  |  Page 6  |  Page 7  |  Page 8  |  Page 9  |  Page 10  |  Page 11  |  Page 12  |  Page 13  |  Page 14  |  Page 15  |  Page 16  |  Page 17  |  Page 18  |  Page 19  |  Page 20  |  Page 21  |  Page 22  |  Page 23  |  Page 24  |  Page 25  |  Page 26  |  Page 27  |  Page 28