AURION IMMUNOGOLD REAGENTS EDITION VI ORDERING INFORMATION


zzz Immunogold Reagent Kits (continued)


zzz Conventional Immunogold Double Labeling Kits


NEW!


Successful immuno-detection experiments depend upon the use of high- quality products that, used in combination, yield optimum results. Aurion reagent kits are specifically designed for this purpose.


Now you also have this option for double labeling experiments with this new series of kits containing different combinations of secondary antibody gold reagents commonly used in double labeling experiments. With the addition of Aurion blocking reagent and Aurion BSA-c (10%) incubation buffer additive, these kits have all you need to achieve the best signal-to- noise ratio possible.


z For postembedding labeling z Monodisperse particle population z Minimal size variation and overlap


z Coefficient of variance: <12% for the 6 and 25 nm conjugates and <10% for the 10 and 15 nm conjugates


z Matching and synergistic products z High signal to noise ratio z For the labeling of 400-800 grids


Conventional Immunogold Double Labeling Kits contain: • 1 x 1.0ml 6nm ImmunoGold Reagent • 1 x 1.4ml 15nm ImmunoGold Reagent • 30ml Blocking Solution • 30ml BSA-c


For ease of discrimination between the two antigenic sites, the combination of 6nm and 15nm gold nanoparticles has been depicted. A recent example of the combined use of Aurion 6nm and 15 nm gold conjugates can be found in: Munoz O., et al. (2022) Active PD-L1 incorporation within HIV virions functionally impairs T follicular helper cells. PLOS Pathogens 18(7); e1010673.


zzz 2nm Immunogold Reagent Kits


AURION 2nm Immuno Gold Reagents in the 2nm kits are prepared using a monodisperse size population of 2nm gold nanoparticles. Due to its small gold nanoparticle size, labeling density with AURION 2nm Immuno Gold Reagents is significantly higher compared to particle density obtained with Conventional Immuno Gold Reagents. This makes the conjugate especially suited for detection of low copy numbers of antigen.


The AURION 2nm kits are available in two versions: one for Light Microscopy and one for Electron Microscopy.


z Monodisperse size population z Coefficient of variance < 15% z High labeling density z Suitable for low copy numbers of antigen z For pre- and post embedding


z For light microscopy with Aurion R-Gent SE-Intense


z For electron microscopy with Aurion R-Gent SE-EM


z Direct visualization in EM optional EM Kit


4–8˚C 4–8˚C


LM Kit


4–8˚C 4–8˚C


The Immuno Gold Silver Staining kit for 2nm reagents contains: • 1 small package vial of 2 nm Immuno Gold Reagent • 30 ml AURION BSA-c™ (10%)


• 30 ml AURION R-Gent SE-EM, silver enhancement reagents for Electron Microscopy or 60 ml AURION R-Gent SE-Intense, silver enhancement reagents for Light Microscopy


• 30 ml Blocking solution Cat. No. Description


LM Kit containing 0.4 ml 2nm ImmunoGold Reagent 30 ml Blocking Solution, 30 ml BSA-c and 60 ml R-Gent SE-Intense 25550-71 LM Kit Goat-anti-Rabbit IgG (H&L) 2nm 25550-72 LM Kit Goat-anti-Mouse IgG (H&L) 2nm


EM Kit containing 0.4 ml 2nm ImmunoGold Reagent, 30 ml Blocking Solution, 30 ml BSA-c and 30 ml R-Gent SE-EM 25550-73 EM Kit Goat-anti-Rabbit IgG (H&L) 2nm 25550-74 EM Kit Goat-anti-Mouse IgG (H&L) 2nm


Qty.


Kit Kit


Kit Kit


21


4–8˚C 4–8˚C 4–8˚C


4–8˚C


4–8˚C 4–8˚C 4–8˚C


Pre-embedding double labeling of GFAP (small particles) and synaptophysin (large particles) in brain tissue using two ultrasmall gold conjugates and silver enhancement. Courtesy Mrs. Hong Yi, Emory University Atlanta


Cat. No. Description Qty.


Conventional Double Labeling kit containing 1.0 ml 6 nm Immuno Gold Reagent, 1.4 ml 15 nm Immuno Gold Reagent, 30 ml Blocking Solution and 30 ml BSA-c 25550-73 EM Kit Goat-anti-Rabbit IgG (H&L) 2nm 25550-74 EM Kit Goat-anti-Mouse IgG (H&L) 2nm


25552-01 Double Labeling Kit Goat-anti-Rabbit IgG (H&L) 6nm / Goat-anti-Mouse IgG (H&L) 15 nm


25552-02 Double Labeling Kit Goat-anti-Mouse IgG (H&L) 6nm / Goat-anti-Rabbit IgG (H&L) 15 nm


25552-03 Protein A 6nm / Protein B 15 nm


25552-04 Double Labeling Kit Donkey-anti-Rabbit IgG (H&L) 6nm / Goat-anti-Mouse IgG (H&L) 15 nm


25552-05 Double Labeling Kit Donkey-anti-Mouse IgG (H&L) 6nm / Goat-anti-Mouse IgG (H&L) 15 nm


Kit Kit


Kit Kit


Kit Kit Kit


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