AURION IMMUNOGOLD REAGENTS EDITION IV ORDERING INFORMATION
TECHNICAL TIPS... Reactivity of Protein A and Protein G
Membrane labeling with protein A 15 nm gold conjugate. Courtesy of T.Wakefield, University of Auburn, Alabama Species
Rabbit Mouse
Ig(sub)class Ig
IgG1
IgG2a IgG2b IgG3 IgA IgM
Rat IgG1
IgG2a IgG2b IgG2c IgM
Man Goat Sheep
Guinea Pig Chicken Pig
Horse Cow Dog
IgG1 IgG2 IgG3 IgG4 IgA IgM IgD Ig Ig Ig Ig Ig Ig Ig Ig
Protein A ++ -
+ + + - - - - -
+ -
++ ++ -
++ -
+ - - -
++ -
++ -
+ +
Protein G ++ + + +
++ ? ? ?
++ + + ?
++ ++ ++ ++ - - -
++ ++ + -
+
++ ++ +
F(ab’)2 Fragment of Goat-anti-Rabbit lgG (H&L) Particle Size
4–8˚C 4–8˚C 4–8˚C 4–8˚C 4–8˚C
Ultra-Small
EM-grade 6nm EM-grade 10nm EM-grade 15nm EM-grade 25nm
Cat # 25360 25363 25362 25366 25368
4–8˚C 4–8˚C 4–8˚C 4–8˚C 4–8˚C
Ultra-Small
EM-grade 6nm EM-grade 10nm EM-grade 15nm EM-grade 25nm
Cat # 25370 25373 25372 25376 25378
4–8˚C 4–8˚C 4–8˚C 4–8˚C 4–8˚C
Ultra-Small
EM-grade 6nm EM-grade 10nm EM-grade 15nm EM-grade 25nm
Cat # 25380 25383 25382 25386 25388
4–8˚C 4–8˚C
Ultra-Small EM-grade 10nm
Cat # 25410 25398
Size(ml) 1.5 2.5 2.5 3.5 3.5
Size(ml) 1.5 2.5 2.5 3.5 3.5
Size(ml) 1.5 2.5 2.5 3.5 3.5
Size(ml) 1.5 2.5
Cat # 25361 25364 25365 25367 25369
Cat # 25371 25374 25375 25377 25379
Cat # 25381 25384 25385 25387 25389
Cat # 25411 25399
1.0 1.0 1.4 1.4
F(ab’)2 Fragment of Goat-anti-Mouse lgG (H&L) Particle Size
1.0 1.0 1.4 1.4
F(ab’)2 Fragment of Goat-anti-Mouse lgG+lgM Particle Size
1.0 1.0 1.4 1.4
F(ab) Fragment of Sheep-anti-Digoxigen Particle Size
Size(ml) .6
1.0
Size(ml) .6
Size(ml) .6
4–8˚C 4–8˚C
F(ab’) Fragment of Goat-anti-Rabbit lgG (H&L) Particle Size
Ultra-Small Ultra-Small
Cat # 25412
Cat # 25414
Size(ml) 1.5
Size(ml) 1.5
Cat # 25413
Cat # 25415
Size(ml) .6
F(ab’) Fragment of Goat-anti-Mouse lgG (H&L) Particle Size
F(ab’) Fragment of Goat-anti-Rat lgG (H&L) Particle Size
4–8˚C Ultra-Small
Cat # 25416
4–8˚C
4–8˚C 4–8˚C 4–8˚C 4–8˚C
Ultra-Small
EM-grade 6nm EM-grade 10nm EM-grade 15nm EM-grade 25nm
Cat # 25420 25423 25428 25432 25435
Size(ml) 1.5
Rabbit-anti-Chicken lgG (H&L) Particle Size
Size(ml) 1.5 2.5 2.5 3.5 3.5
Cat # 25417
Cat # 25421 25424 25429 25433 25436
zzz Cross Adsorbed Products
Cross reactivity can be a problem when working with primary antibodies sourced from animal species that are evolutionary closely related to the source of tissues or cells that antigens are to be detected in.
An example is using a mouse monoclonal on rat tissue. With many monoclonal antibodies of mouse origin and some of rat, and the prevailing animal cells and tissues being used in studies likewise originating from rat or mouse, it often happens that cross reactivity occurs, leading to false positive results.
A second problem that derives from cross-reactivity occurs in double labeling when using primary antibodies from closely related animal sources. With this in mind, Aurion has developed two series of new conjugates:
Size(ml) .6
4–8˚C 4–8˚C
Goat-anti-Mouse IgG (H+L)xRat (cross adsorbed against Rat immunoglobulins)
Particle Size
EM-grade Ultra Small EM-grade 10nm
Cat # 25900 25902
Size(ml) 1.5 2.5
Cat # 25901 25903
Size(ml) 0.6 1.0
Goat-anti-Rat IgG (H+L)xMouse (cross adsorbed against Mouse immunoglobulins)
Particle Size
4–8˚C 4–8˚C
EM-grade Ultra Small EM-grade 10nm
Cat # 25904 25906
Size(ml) 1.5 2.5
Cat # 25905 25907
Size(ml) 0.6 1.0
Secondary antibodies have been thoroughly purified using solid phase technique. These purified antibodies allow detection of antigens with mouse monoclonal antibodies in rat tissue or rat monoclonal antibodies in mouse tissue, without causing interfering binding to either endogenous mouse or rat immunoglobulins.
The antibodies can be used for double labeling as well when both mouse and rat primary antibodies are used with other than mouse or rat tissue.
Please note:
Cross adsorption against closely related species may result in epitope recognition with reduced binding force and lower labeling density.
EMS and Aurion would like to inform you that the strongest labeling intensity is usually achieved with the least refined antibodies. Users are advised to take this into account when choosing a product from the Aurion range of anti-mouse or anti-rat conjugates. In case of doubt, our technical staff will gladly assist you in making the best choice.
15
Size(ml) .6
Size(ml) .6
Size(ml) .6
1.0 1.0 1.4 1.4
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