AURION IMMUNOGOLD REAGENTS EDITION VI PRODUCT INFORMATION


zzz 2nm Gold Nanoparticle Conjugated Goat- anti-Rabbit IgG and Goat-anti-Mouse IgG


This new series of immunogold reagents have the capacity to fill the gap between Aurion's unique ultra-small gold reagents and the conventional sized 6-25nm gold conjugates.


Features z Monodisperse size population z Coefficient of variance < 15% z For pre- and post-embedding labeling z Direct visualization in EM optional


Product Description


Aurion 2nm conjugated GAR and GAM IgG are prepared using a monodisperse size population of 2nm gold nanoparticles. Due to its small gold nanoparticle size, labeling density with these Aurion 2nm Immuno Gold Reagents is significantly higher compared to particle density obtained with comparable Conventional Immuno Gold Reagents. This makes these 2nm immunogold conjugates especially suited for detection of low copy numbers of antigen.


Aurion 2nm Immunogold Reagents contain 60-80 µg of specific protein/ml for IgG conjugates. The average gold particle diameter is 2nm.


The reagents are supplied in PBS with 1% Bovine Serum Albumin and 15 mM NaN3. The activity of each lot is determined using a dot-spot test system as described by Moeremans et al., J. Immunol. Methods, 74, (1984), 353.


The products are available in two package sizes: 0.4 ml for 320 grids or 160 slides 1.0 ml suited for 800 grids or 400 slides


Specificity


AURION 2nm Immunogold Reagents are prepared using the highest quality antibodies or binding agents available. Antibodies are immuno affinity purified and immuno cross-adsorbed to reduce non-specific reactions.


Conjugated to Goat anti Rabbit IgG (H&L) and Goat anti Mouse IgG (H&L) For other species and specificities we kindly refer you to our Custom Labeling Service.


Storage


AURION 2nm Immuno Gold Reagents have a guaranteed shelf life of 18 months from the date of quality control analysis.


The products should be stored at 4-8°C. Freezing is not recommended.


Typical size distribution of AURION 2nm Immuno Gold particles


TECHNICAL TIPS... Application Instructions


Detailed information is provided on the package inserts.


On-Grid Labeling


For most applications grids are floated on top of drops of dilute reagent displayed on a sheet of Parafilm™. The use of gold or nickel grids is recommended.


Whole mount and intact cell labeling Specimens are kept floating in dilute reagent on a rocking table.


Recommended Incubation Solution: PBS,


Post embedding immunolabeling: Detection of Lamp2 on Tokuyasu sections of 4% PFA fixed HeLa cells using AURION GAM IgG 2nm and AURION R-Gent SE-EM.


Courtesy of M. Mari, UMCG & RUG, Molecular Cell Biology, Groningen, The Netherlands


Pre-embedding labeling on 2%PFA/GA fixed and freeze thaw permeabilized organotypic cultured brain slices using rabbit anti GFP, AURION GAR 2nm and R-Gent SE-EM.


Courtesy of Y. Sun, D. Guerrero-Given and N. Kamasawa; EM Core Facility, Max Planck Florida, Institute for Neuroscience, Jupiter, FL USA


(10 mM Phosphate buffer, 150 mM NaCl), 0.1-0.2 % AURION BSA-C™ 15 mM NaN3 pH 7.4


Additional Information


For additional information, please see the Aurion Immunogold Newsletters and Technical Tips on our website.


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z Homogeneous silver enhancement z In EM applications; use R-Gent SE-EM z For LM/bio-assays use R-Gent SE-Intense


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