AURION IMMUNOGOLD REAGENTS EDITION VI PRODUCT INFORMATION
zzz Gold Nanoparticles – Carboxyl Functionalized
We are proud to announce the introduction of a series of Carboxyl-functionalized Gold Nanoparticles. This product has been developed to facilitate conjugating gold nanoparticles to molecules that cannot be conjugated via the classic direct adsorption method. The product is especially suited for covalent conjugation of small ligands.
Features z Carboxyl (COOH) functionalized
z Particle sizes 6, 10, 15 and 25nm z Bind to primary amino amines in target molecule z Conjugation using EDC/sulfo-NHS chemistry z Recommended for conjugation of small ligands z Detailed conjugation protocol z Ready and easy to use, long term stability
Introduction TECHNICAL TIPS...
Application Instructions Detailed information is provided in the package inserts.
Storage
Aurion Gold Nanoparticles (Carboxyl Functionalized) have a guaranteed shelf life of 12 months from the date of quality control analysis.
The products should be stored at 4-8°C. Freezing is not recommended.
Aurion Gold Nanoparticles (Carboxyl Functionalized) are polyethylene glycol-carboxyl stabilized gold nanoparticle solutions based on defined particle sizes of 6, 10, 15 and 25nm.
They are prepared using in-house developed production protocols giving the product a narrow size dis- tribution, long term stability and optimal conjugation properties. Biomolecules having primary amines available can be covalently conjugated using EDC/sulfo-NHS chemistry.
Product Description
Biomolecules that are too small to be conjugated to Gold Nanoparticles via adsorption can be covalent- ly linked to Carboxyl-functionalized Gold Nanoparticles. Primary amines are present in e.g. the N-termi- nal side of peptides and in the side group of the amino acid lysine. The conjugation relies on well known and proven EDC/sulfo-NHS chemistry. EDC (N-(3-Dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride) is a water soluble carbodiimide which transforms the carboxyl groups on the gold to an active ester in the presence of sulfo-NHS (N-Hydroxy-sulfosucciimide, sodium salt). These sulfo-NHS esters are relatively stable in acidic environment and couple rapidly to the amine(s) in the target molecules.
Aurion Gold Nanoparticles (Carboxyl Functionalized) are prepared according to a unique protocol, war- ranting narrow size distribution, long term stability and optimal conjugation properties. They are avail- able in size ranges 6, 10, 15 and 25 nm. The particle population is monodisperse and thus shows minimal size variation and overlap. Typically, the coefficient of variance for the 6 and 25 nm particle size sols is less than 12%, whereas the 10 and 15 nm size sols show less then 10% variation. Actual lot specifications (size, variation and expiry date) are indicated on the accompanying package insert.
Aurion Gold Nanoparticles (Carboxyl Functionalized) are supplied in 10mM MES buffer, pH 5.0. Package size: 4x5 ml of high quality Carboxyl-functionalized Gold Nanoparticles at an OD520nm = 1.0.
Particle size and size distributions of the AURION Gold Nanoparticles – Carboxyl Functionalized
Detection of endogenous biotin on formalin fixed rat kid- ney tissue. After antigen retrieval in 10mM citrate buffer, pH 6, biotin was detected with streptavidin and visualized with 10nm gold conjugated biotin and silver enhancement. Positive signal (top) is mainly present in epithelial cells of proximal tubules. (Bottom) Negative control.
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6 nm
10 nm
15 nm
25 nm
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