MICROSCOPY & IMAGING
Fig. 2 (c) Raman
spectra of the fatty phases (red: normal
butter, green: spreadable butter) with zoom-in
the C=C stretching mode (approximately 1,655 cm-1
(approximately 1,444 cm-1 ) and the CH2 and this
ratio was higher for the spreadable butter than for the conventional sample. Tis indicates a higher amount of unsaturated fatty acids in the spreadable butter, which likely contributes to the improved spreadability. Fat-spreads with a high amount of unsaturated amino acids and a reduced fat content are also considered healthier than conventional butter.
scissoring mode )[2]
3D confocal Raman imaging is a powerful analytical tool for nondestructively characterising the molecular composition of sample volumes, as illustrated by emulsion measurements. For generating high- quality 3D chemical images, a Raman microscope that simultaneously provides high spatial resolution and confocality, signal sensitivity and acquisition speed is required.
SUMMARY
References [1] T. Dieing (2018). In: J. Toporski, T. Dieing, O. Hollricher (eds.) Confocal Raman Microscopy. Springer Series in Surface Sciences 66, pp. 121 – 153. Springer, Cham. DOI: 10.1007/978-3-319-75380-5_6 [2] K. Czamara et al. (2014) J. Raman Spectrosc. 46: 4 – 20. DOI: 10.1002/jrs.4607
Thomas Dieing, Damon Strom & Eleni Vomhof are with WITec.
https://raman.oxinst.com
ALL-IN-ONE SOLUTION FOR WIDEFIELD AND CONFOCAL IMAGING C
restOptics, a manufacturer of high-end microscopy solutions and advanced systems for
fluorescence microscopy and diagnostic applications, has announced the launch of its new spinning disc confocal microscope system, Cicero. Focusing on the essential elements of epi-fluorescence and confocal microscopy, Cicero has been developed to make high-end imaging accessible to the wider scientific community by providing a compact and cost-effective system for powerful imaging solutions. The new system is an all-in-one solution
for widefield and confocal imaging. Building on the recent success of the company’s DeepSIM module the new product has been developed to expand the company’s portfolio and enable accessible fluorescence imaging within one compact, budget- friendly, yet high-performing solution. The spinning disc confocal instrument can be easily integrated into existing workflows, with maximum configuration
Cortical organoid captured using Cicero
flexibility to support a seamless and user friendly transition, enabling a broad range of imaging applications across basic and applied research. The compact system facilitates
streamlined transition between widefield and confocal imaging, enabling users to simply switch between modes as their
imaging requirements evolve. Widefield mode supports informative data collection from samples, such as cell monolayers and tissue sections, whereas the confocal mode allows higher-quality analysis of larger 3D structures, including organoids or whole organisms. Additionally, Cicero’s speed and light efficiency provides the capability for prolonged live imaging and the capture of fast cellular events, such as chromosome segregations and organelle trafficking. Alessandra Scarpellini, chief commercial
officer, CrestOptics, said “We realised that there was a need for an instrument that was equally powerful and easy to integrate into different platforms. Our team have worked hard to ensure that Cicero retains the level of technological excellence and performance that we provide in our existing portfolio, whilst ensuring that the instrument was compact and cost-effective.”
For more information visit
www.crestoptics.com
www.scientistlive.com 57
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