CHROMATOGRAPHY


COMPREHENSIVE MYCOTOXIN ANALYSIS


Dr Shun-Hsin Liang & Dr Jamie York explore simultaneous determination of alternaria toxins, ergot alkaloid epimers and other major mycotoxins in various food matrices by LC-MS/MS


A


s more mycotoxins come under regulatory purview, new methods are needed to help food safety labs continue to operate efficiently.


Comprehensive multi-mycotoxin methods are an attractive alternative to separate methods for different analyte lists, but they can be difficult to develop due to the wide range of chemical characteristics among mycotoxin classes. In particular, the alternaria toxins and ergot alkaloids create additional challenges for method developers. Tese emerging mycotoxins are unique in that, when analysed on a C18 column, high pH conditions must be used to obtain acceptable peak shape for the Alternaria toxins and for adequate separation of the ergot alkaloid epimers. Use of high pH conditions is stressful for LC columns and not suitable for analysing other classes of mycotoxins, so another approach is required for a truly comprehensive method.


Another factor that complicates any comprehensive method is the


New methods are required for successful mycotoxin analysis


26 www.scientistlive.com


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