MICROSCOPY & IMAGING
OPTICAL SECTIONING DEVICE FOR HIGH-CONTRAST IMAGING T
he new Slideview VS200 v. 4.1 software supports a host of new features, including the SILA optical sectioning device that enables users to obtain high-contrast images from deep within their sample. The SILA optical sectioning device
uses speckle illumination combined with HiLo microscopy to achieve high-contrast images. This camera-based technology
quickly captures two illuminated images that are then mathematically processed to remove out-of-focus light. Unlike some techniques, this method is simple for anyone to learn and requires no special calibration. Slideview v. 4.1 software guides users to
acquire optical sectioning images. Optical sectioning capabilities remain the same as you image deeper, enabling users to capture stunning images from deep within their samples. The SILA
optical sectioning device is easy to add to any existing VS200 system, including those with a slide loader. It is compact and attaches to the scanner’s fluorescence
illuminator. Once attached, the optical sectioning device works with most sample types, including cleared and fixed cells and tissues thicker than 100 microns as well as any magnification. Current detection methods can’t always identify transparent and morphologic features on a sample and can miss critical targets, but TruAI technology – now supported in Slideview v. 4.1 software – can accurately segment these features and differentiate them from other similar- looking features. To reduce the data burden, TruAI uses
its selective scanning mode to identify the sample area and skips scanning areas that are not of interest. This optimises data management, including storage, uploading and sharing images.
Users can easily upload images to the
database, differentiate between users and take advantage of offline visualisation and annotation tools.
For more information visit
www.olympus-lifescience.com
NEW PIEZO OBJECTIVE SCANNER ANNOUNCED N
anopositioning expert, Queensgate, has announced the launch of the Nano Scan OP800 piezo objective scanner. Its mechanical design together with integral capacitive feedback sensors offer users leading positioning accuracy, while its stiff construction allows the standard selectable tuning pre-sets to support a range of objectives from 150g to 500g. This makes it highly versatile for use in a wide range of applications in life sciences and industry. The OP800 offers 800 µm of closed-loop travel range for easier operation within the piezo focus range without adjusting the sample. It also allows deeper Z-stacks to be completed: ideal for multiphoton imaging techniques. Auto-calibration on power-up automatically
offsets the range to correct for load and environmental changes to maintain the full 800 µm travel. It is flexure-guided with built-in
capacitive position feedback. This provides high linearity of motion and market- leading repeatability of just 5nm, ensuring consistency between multiple Z stacks. Small rotational errors help ensure the full field of view stays in focus.
Images are captured rapidly thanks to the
fast step-settle times. Constant velocity mode operation allows rapid precision ramping to form very quick image stacks over long ranges. This is several times faster than conventional position control can achieve. As is standard with Queensgate stages, the OP800 offers true plug-and-play capability without the need to match controllers. Both digital and analogue interfacing are easy to set up, while the low-noise, high- powered digital electronics allow rapid stepping. The OP800 is suitable for use in life sciences, materials and semiconductor applications, such as optical sectioning, surface analysis, wafer inspection and scanning interferometry.
For more information visit
www.nanopositioning.com
www.scientistlive.com 55
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