each AS GC confi guration


AS confi guration in Chromeleon CDS


AI/AS 1610 Gemini: easy confi guration path


Sample sequence setup in Chromeleon CDS


Front and back fl ow paths can have diff erent components


Two AS are set up in the instrument confi guration page


Two sample sequences are set up, one for each channel (front/back)


AI/AS 1610 Gemini: easy confi guration path Hardware setup In the Gemini confi guration, regardless of the operational mode, the two autosamplers are placed on the front and back injectors by means of a dedicated mounting bracket with a quick and easy slide-in installation and self-alignment on the inlet port and the vials. The setup is further simplifi ed with power supply, communication, and handshaking carried out through a single-cable connection between each autosampler and the GC system. Either in a single tower or Gemini confi guration, the design of the AI/ AS 1610 autosampler allows for an easy access to the inlet port, facilitating maintenance operations. This is achieved through a sliding support to easily move aside the tower from the injector (Figure 3).


Hardware setup In the Gemini confi guration, regardless of the operational mode, the two autosamplers are placed on the front and back injectors by means of a dedicated mounting bracket with a quick and easy slide-in installation and self-alignment on the inlet port and the vials. The setup is further simplifi ed with power supply, communication, and handshaking carried out through a single-cable connection between each autosampler and the GC system. Either in a single tower or Gemini confi guration, the design of the AI/AS 1610 autosampler allows for an easy access to the inlet port, facilitating maintenance operations. This is achieved through a sliding support to easily move aside the tower from the injector (Figure 3).


Confi guration in Chromeleon CDS


Gemini confi guration in Chromeleon CDS is carried out differently according to the operation mode.


Under High-throughput mode, two autosamplers need to be confi gured, corresponding to the ‘Front’ and ‘Back’channels, setting the same IP address and specifying which com port is used to connect to the GC. When using the Confi rmation mode, a single autosampler needs to be confi gured. Its method parameters will be fully shared between the two autosamplers.


Confi rmation mode data review


When working in Confi rmation mode, data obtained from the same sample on the two channels can be easily visualised for direct comparison. Data originating from two parallel channels are collected within a single data fi le, facilitating the data review. This analytical mode is particularly useful for comparing two different detector types having different characteristics in terms of sensitivity and selectivity, or comparing separations achieved on two different analytical columns for identifi cation confi rmation. Figure 4 shows an example of the same standard mixture simultaneously analysed on two channels, with different selective detectors as FID and ECD, and the easy comparison of the data from the two channels.


Confi rmation mode data review When working in Confi rmation mode, data obtained from the same sample on the two channels can be easily visualized for direct comparison. Data originating from two parallel channels are collected within a single data fi le, facilitating the data review. This analytical mode is particularly useful for comparing two diff erent detector types having diff erent characteristics in terms of sensitivity and selectivity, or comparing separations achieved on two diff erent analytical columns for identifi cation confi rmation. Figure 4 shows an example of the same standard mixture simultaneously analyzed on two channels, with diff erent selective detectors as FID and ECD, and the easy comparison of the data from the two channels.


Extended applicability of the AI/AS 1610


The AI/AS 1610 liquid autosampler meets the highest requirements of ruggedness, reliability, and ease of use and fulfi ls the needs of high-throughput testing laboratories. New operational capabilities are available to expand the applicability in analytical testing laboratories and increase reliability, robustness, and productivity.


Safe operation and improved usability are guaranteed by:


• The automatic selection of the best injection settings through recognition of the injector type and injection mode


Figure 3. Design of the AI/AS 1610 autosampler. Easy access to the inlet port is achieved through a sliding support.


Easy switch from dual to single mode operation


When the High-throughput mode is confi gured, switching from dual to single mode operation is possible by simply fl agging the dedicated option in the Instrument pane: each autosampler, either front or back, can be employed in a single-autosampler operation to run sequences on a single channel without changing hardware settings or Chromeleon confi guration.


Figure 3. Design of the AI/AS 1610 autosampler. Easy access to the inlet port is achieved through a sliding support.


Easy switch from dual to single mode operation When the High-throughput mode is confi gured, switching from dual to single mode operation is possible by simply fl agging the dedicated option in the Instrument pane: each autosampler, either front or back, can be employed in a single-autosampler operation to run sequences on a single channel without changing hardware settings or Chromeleon confi guration.


3 Untitled-7 3 Conclusions


The Gemini confi guration provides a simple and cost-effective solution to increase laboratory throughput. The simultaneous injections in two channels for unattended analyses of up to 310 samples is achieved with easy-on/off set up and connectivity thanks to the usage of a single, multi-purpose connection between each autosampler and the GC. High-throughput mode or Confi rmation mode are easily enabled through Chromeleon CDS, which facilitates the monitoring of multiple parallel sequences on the same GC as well as multiple systems within the same laboratory.


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• Easier syringe viewing and replacement thanks to the illuminated syringe compartment • Facilitated syringe scale reading through a built-in magnifying lens • At-a-glance monitoring with enhanced colour-coded instrument status indication


The AI/AS 1610 autosampler offers the fl exibility to cover a wide variety of applications, methods, and samples thanks to enhanced sample handling capabilities and high- throughput features:


• Minimised injection-to-injection cycle time with pre-injection operations performed in advance the GC ready conditions


• Expanded applicability with large sample volume injections up to 80 µL • Optimised solvent consumption with modifi able syringe washing volumes • Optimised solvent washing capabilities with the selection of up to four different solvents


• Optimised viscous samples management with selectable sample drawing speed (Fast/ Slow) and programmable viscosity delay (0–7 s)


• High sampling fl exibility with two-layer injection mode, with layers and air gaps user defi nable volumes (for sandwich injection, internal standard addition, or in-needle derivatisation)


each AS


Front and back fl ow paths can have diff erent components


One AS is set up in the instrument confi guration page


Enhanced sequence operations with Chromeleon CDS


One sample sequence is set up, with two channel acquisition for each sample type


Chromeleon CDS is a powerful chromatography data system software for qualitative and quantitative applications combining chromatography and MS workfl ows in a very intuitive and productive way.


Confi guration in Chromeleon CDS Gemini confi guration in Chromeleon CDS is carried out diff erently according to the operation mode.


Chromeleon CDS 7.3 offers additional features to streamline sequence operations. Those functionalities are of particular interest for users running multiple parallel instruments within the same laboratory, such as in the case of several Gemini confi gurations.


• The instrument status visualisation is facilitated by a new Overview tab window displaying all instruments and their status within a single view (Figure 5).


Under High-throughput mode, two autosamplers need to be confi gured, corresponding to the ‘Front’ and ‘Back’ channels, setting the same IP address and specifying which com port is used to connect to the GC. When using the Confi rmation mode, a single autosampler needs to be confi gured. Its method parameters will be fully shared between the two autosamplers.


• Streamlined sequences start is achieved with just two clicks from the Overview tab. Also, it is possible to launch eWorkfl ows and view instrument queues from each line of the tab.


• Enhanced operativity with the Detach View function allows the Overview tab to be visualised as an independent fl oating window. The user can thus perform other operations on the screen (e.g., data processing) while keeping an eye on the status of the instruments of the lab.


• The monitoring of selected instruments is facilitated by setting them as ‘Favorites’ under Instrument view of the Chromeleon Console. In a laboratory with many instruments, this allows visualisation of only those that are worth monitoring.


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Figure 5. Screenshot of the Overview tab in Chromeleon CDS 7.3. Lines highlighted in green refer to a system running samples on both injectors in High-throughput mode.


Additional usability features and benefi ts of AI or AS 1610 liquid autosamplers in single tower or Gemini confi guration, in combination with the TRACE 1600 Series GC are key to streamlining daily operations and providing what is required in a high-throughput laboratory for liquid sample injection automation.


Find out more at thermofi sher.com/aias


Figure 4. Analysis using Gemini Confi rmation mode. A standard mixture containing dichlorobenzene (DCB), dibutylsulfi de (DBS), and nitrobenzene (NB) is analysed using GC-ECD (top) and GC-FID (bottom). Data obtained on the two parallel channels are collected within a single data fi le and the results can be visualised by clicking on the respective chromatogram (here the front channel chromatogram is selected). Processing methods can be applied to both channels or single channels only.


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