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Figure 2. Thermo Scientifi c TRACE 1610 GC equipped with (A) Gemini AI 1610 (total of 8-vial capacity); (B) Gemini AS 1610 (total of 310-vial capacity).


Figure 2. Thermo Scientific™ Goal


To illustrate the simplifi ed usability of the Thermo Scientifi c™ AI/AS 1610 autosampler in the Gemini confi guration to meet high-throughput demand for analytical testing laboratories.


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A confi dent automation of liquid sample injection in a GC or GC-MS through a robust, reliable, and easy-to-use autosampler is what a high-throughput testing laboratory needs to respond to a heavy workload. The enhanced usability of the Thermo Scientifi c AI/AS 1610 liquid autosampler (Figure 1) makes this platform the fi t-for-purpose solution, combining operational simplicity with injection mode fl exibility. In order to support analytical testing laboratories facing high sample throughput demand, the dual-tower AI/AS 1610 Gemini confi guration allows for simultaneous injection on two channels, doubling the throughput achievable on a single GC system, or confi rming the same sample on two simultaneous runs.


This Technical Note summarises the main features of the Gemini confi guration in combination with the Thermo Scientifi c™ Chromeleon™ Chromatography Data System (CDS) version 7.3, describing the different ways of operations.


Figure 1. (A) AI 1610 autoinjector with 8-vial capacity; (B) AS 1610 autosampler with 155-vial capacity.


A Scientifi c™ B Chromeleon™ Chromatography Data System (CDS) version 7.3, describing the diff erent ways of operations. B TRACE™ AI/AS 1610 Gemini operational modes


AI/AS 1610 Gemini operational modes In the Gemini configuration, two AI 1610 autoinjectors (with a total of 16 vials capacity) or two AS 1610 autosamplers (with a total of 310 vials capacity) can operate at the same time on the Thermo Scientific™


TRACE™ 1600 Series GC,


In the Gemini confi guration, two AI 1610 autoinjectors (with a total of 16 vials capacity) or two AS 1610 autosamplers (with a total of 310 vials capacity) can operate at the same time on the Thermo Scientifi c™ TRACE™ 1600 Series GC, each using a dedicated injector (Figure 2). The system is fully controlled by Chromeleon CDS, which offers a series of features to streamline the operations in Gemini mode.


each using a dedicated injector (Figure 2). The system is fully controlled by Chromeleon CDS, which offers a series of features to streamline the operations in Gemini mode.


The Gemini configuration allows operation in two different modes, which display some key differences according to the way they exploit the Gemini setup (Table 1).


The Gemini confi guration allows operation in two different modes, which display some key differences according to the way they exploit the Gemini setup (Table 1).


High-throughput mode: In this modality, the system is considered as composed of two autosamplers separately confi gured. Autosampler methods can be the same for the two channels or they can vary: in both cases, sample handling is synchronised in such a way the injections take place simultaneously. The injections follow two separate sample sequences, with data acquisition and processing achieved separately. The two channels (inlet, column, detector) may also have different components. The only obvious limitation is that the GC oven program needs to be the same in the two methods. This mode is generally used to support processing of large batches of samples, and therefore the two sample trays are typically loaded with different samples.


• High-throughput mode: In this modality, the system is considered as composed of two autosamplers separately configured. Autosampler methods can be the same for the two channels or they can vary: in both cases, sample handling is synchronized in such a way the injections take place simultaneously. The injections follow two separate sample sequences, with data acquisition and processing


• Confirmation mode: In this modality, the injector, column, and detector of the two channels can still be different, but a single autosampler is configured for both the flow paths, so that the autosampler method is the same for the two channels. This mode is typically used to run the same sample on two channels for confirmation purposes. The uniqueness of this operation mode lies in the fact that data obtained from the two channels running simultaneously are stored in a single data set, thus simplifying direct comparison of the results. Typically, a given position in both trays is occupied by vials containing the same sample.


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Confi rmation mode: In this modality, the injector, column, and detector of the two channels can still be different, but a single autosampler is confi gured for both the fl ow paths, so that the autosampler method is the same for the two channels. This mode is typically used to run the same sample on two channels for confi rmation purposes. The uniqueness of this operation mode lies in the fact that data obtained from the two channels running simultaneously are stored in a single data set, thus simplifying direct comparison of the results. Typically, a given position in both trays is occupied by vials containing the same sample.


2 08/03/2022 13:11 Table 1. Main differences between the AI/AS 1610 Gemini operation modes


Table 1. Main diff erences between the AI/AS 1610 Gemini operation modes High-throughput mode


Sample location Injection settings GC confi guration


Figure 1. (A) AI 1610 autoinjector with 8-vial capacity; (B) AS 1610 autosampler with 155-vial capacity.


Figure 1. (A) AI 1610 autoinjector with 8-vial capacity; (B) AS 1610 autosampler with 155-vial capacity.


Scientifi c™ Chromeleon™ Chromatography Data System (CDS) version 7.3, describing the diff erent ways of operations. 08/03/2022 13:11


AS confi guration in Chromeleon CDS


Sample sequence setup in Chromeleon CDS


Samples are independently placed on AS tray


Diff erent injection methods can be used on each AS


Front and back fl ow paths can have diff erent components


Two AS are set up in the instrument confi guration page


Two sample sequences are set up, one for each channel (front/back)


AI/AS 1610 Gemini: easy confi guration path Hardware setup In the Gemini confi guration, regardless of the operational mode, the two autosamplers are placed on the front and back injectors by means of a dedicated mounting bracket with a quick and easy slide-in installation and self-alignment on the inlet port and the vials. The setup is further simplifi ed with power supply, communication, and handshaking carried out through a single-cable connection


Confi rmation mode


Position ‘x’ on each AS tray has an aliquot of the same sample


The same injection method is used on each AS


Front and back fl ow paths can have diff erent components


One AS is set up in the instrument confi guration page


One sample sequence is set up, with two channel acquisition for each sample type


Confi guration in Chromeleon CDS Gemini confi guration in Chromeleon CDS is carried out diff erently according to the operation mode.


Under High-throughput mode, two autosamplers need to be confi gured, corresponding to the ‘Front’ and ‘Back’ channels, setting the same IP address and specifying which com port is used to connect to the GC. When using the Confi rmation mode, a single autosampler needs to


LAB ASIA - APRIL 2022


achieved separately. The two channels (inlet, column, detector) may also have different components. The only obvious limitation is that the GC oven program needs to be the same in the two methods. This mode is generally used to support processing of large batches of samples, and therefore the two sample trays are typically loaded with different samples.


1610 equipped with (a) Gemini AI 1610 (total of 8-vial capacity); (b) Gemini AS 1610 (total of 310-vial capacity) Doubling the sample throughput with AI/AS 1610 Gemini confi guration


Simplifi ed operational modes and Chromeleon CDS control Authors: Andrea Romano, Manuela Bergna, Daniela Cavagnino, Thermo Fisher Scientifi c, Milan, IT


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