18


lipids while maintaining throughput and reproducibility, producing deeper, richer, and more reproducible datasets [16,17].


The final technological component is the optical detector, which Zamboni credits as the “secret sauce” of the ZenoTOF 8600 system for its “fantastic optics” [3]. Combined with Mass Guard technology, it allows high ion current operation while minimising contamination [21]. The Mass Guard technology filters ions before mass separation using T Bar electrodes in the Q0 region, removing contaminants and producing a cleaner sample plume. This reduces matrix effects, even with residue on the source curtain plate, maintaining peak analyser sensitivity up to twice as long and optimising instrument uptime [27,28].


For researchers like Zamboni, ZT Scan DIA 2.0 - leveraging the OptiFlow Pro ion source, QJet ion guide, DJet ion guide, Zeno trap, and new optical detector plus Mass Guard technology - finally enables unbiased, high-throughput metabolomics workflows that can capture the complexity of biology [3,11].


References


1. Chen Y. Li E-M, Xu L-Y. Guide to Metabolomics Analysis: A Bioinformatics Workflow. Metabolites 2022, 12, 357. doi: 10.3390/metabo12040357.


2. Wishart DS. Emerging applications of metabolomics in drug discovery and precision medicine. Nat Rev Drug Discov. 2016;15:473–84. doi: 10.1038/nrd.2016.32.


3. Zamboni N. The portal to multimodal metabolomics and lipidomics. SCIEX YouTube channel. Available at: https://www.youtube.com/watch?v=jT-6LoCbX0c (accessed July 26, 2025).


4. Macauslane KL, Pegg CL, Nouwens AS, Kerr ED, Seitanidou J, Schulz BL. Electron-Activated Dissociation and Collision-Induced Dissociation Glycopeptide Fragmentation for Improved Glycoproteomics. Anal Chem. 2024;96:10986–94. doi: 10.1021/acs.analchem.4c01450.


5. Heiles S. Advanced tandem mass spectrometry in metabolomics and lipidomics-methods and applications. Anal Bioanal Chem. 2021;413:5927–48. doi: 10.1007/s00216-021-03425-1.


6. Zamboni N. On the benefits and role of electron-induced dissociation in lipidomics. Metabolics Blog. Available at: https://metabolomics.blog/2024/04/ead-and-lipidomics/ (accessed August 15, 2025).


7. Wu V, Moyal A, Othman A, Zamboni N. Optimization of electron-induced dissociation parameters for molecular annotation of glycerides and phospholipids in fast LC-MS. ChemRxiv 2025. Available at: https:// chemrxiv.org/engage/chemrxiv/article-details/68208af7927d1c2e661238ab (accessed August 15, 2025).


8. Baker PRS, Proos R. Untargeted data-dependent acquisition (DDA) metabolomics analysis using the ZenoTOF 7600 system. SCIEX Technical Note. Available at: https://sciex.com/tech-notes/life-science- research/metabolomics/untargeted-data-dependent-acquisition--dda--metabolomics-analysi (accessed August 15, 2025).


9. Baba T, Ryumin P, Duchoslav E, et al. Dissociation of Biomolecules by an Intense Low-Energy Electron Beam in a High Sensitivity Time-of-Flight Mass Spectrometer. J Am Soc Mass Spectrom. 2021;32:1964–75. doi: 10.1021/jasms.0c00425.


10. Pearson M, Hunter C, Baba T. Complete structural elucidation of lipids in a single experiment using electron activated dissociation (EAD). SCIEX Technical Note. Available at: https://sciex.com/tech-notes/life-science- research/lipidomics/complete-structural-elucidation-of-lipids-in-a-single-experiment0 (accessed August 15, 2025).


11. Wu V, Causon J, Rutz A, Moyal A, Zamboni N. A novel QTOF with a 30x sensitivity boost enables multimodal metabolomics and lipidomics. ETH Zürich. Poster.


12. SCIEX. SWATH DIA (data independent acquisition). Available at: https://sciex.com/technology/swath- acquisition (accessed August 15, 2025).


13. Proos R. Improved metabolite identification using data independent analysis for metabolomics. SCIEX Technical Note. Available at: https://sciex.com/tech-notes/life-science-research/metabolomics/improved- metabolite-identification-using-data-independent-analys (accessed August 15, 2025).


14. SCIEX. Understanding SWATH Acquisition. Available at: https://sciex.com/videos/Understanding- SWATH-Acquisition (accessed August 15, 2025).


15. SCIEX. SWATH® Acquisition: A Story of Collaboration. Available at: https://sciex.com/cr/landing-pages/ scanningswath (accessed August 15, 2025).


16. SCIEX. Zeno Trap. SCIEX White Paper. Available at: https://sciex.com/content/dam/SCIEX/pdf/ brochures/zeno-trap-whitepaper.pdf (accessed August 15, 2025).


17. Zeno SWATH DIA. SCIEX White Paper. Available at: https://sciex.com/content/dam/SCIEX/pdf/ brochures/white-paper-zeno-swath-dia-website.pdf (accessed August 15, 2025).


18. Chao H-S, Chen G-Y, Hsu L-C, et al. Using precursor ion scan of 184 with liquid chromatography- electrospray ionization-tandem mass spectrometry for concentration normalization in cellular lipidomic studies. Anal Chim Acta. 2017;971:68–77. doi: 10.1016/j.aca.2017.03.033.


19. Löhmann C, Schachmann E, Dandekar T, Villmann C, Becker C-M. Developmental profiling by mass spectrometry of phosphocholine containing phospholipids in the rat nervous system reveals temporo-spatial gradients. J Neurochem. 2010;114:1119–34. doi: 10.1111/j.1471-4159.2010.06836.x.


20. Zamboni Group. Research. ETH Zürich. Available at: https://imsb.ethz.ch/research/zamboni/research. html (accessed August 15, 2025).


21. SCIEX. Extraordinary discoveries demand extraordinary proof. Available at: https://sciex.com/products/ mass-spectrometers/qtof-systems/8600-system (accessed August 12, 2025).


22. Hunter C. Guidance for source tuning on the OptiFlow Pro ion source. Available at: https://community.sciex. com/2021/08/06/guidance-for-source-tuning-on-the-optiflow-pro-ion-source/ (accessed August 15, 2025).


23. SCIEX. Enabling new levels of quantification. SCIEX Technical Note. Available at: https://sciex.com/tech- notes/technology/enabling-new-levels-of-quantification (accessed August 15, 2025).


24. SCIEX. QJet™ Ion Guide Technology in the API 5000™ LC/MS/MS System. SCIEX Product Bulletin. Available at: https://www.jaytee.com/wp-content/uploads/2021/12/QJet-Ion-Technology-API-5000.pdf (accessed August 15, 2025).


25. SCIEX. Enabling new levels of quantification. SCIEX Technical Note. Available at: https://sciex.com/ content/dam/SCIEX/tech-notes/technology/ruo-mkt-02-11886-a/TripleQuad-7500-Technology-Overview_ RUO-MKT-02-11886-A.pdf (accessed August 15, 2025).


26. SCIEX. SWATH® Acquisition: A Story of Collaboration. Available at: https://sciex.com/cr/landing-pages/ scanningswath (accessed August 15, 2025).


27. SCIEX. Setting a new standard for instrument resilience. Available at: https://sciex.com/products/mass- spectrometers/triple-quad-systems/triple-quad-7500-plus-system (accessed August 15, 2025).


28. Lee H, Moore I, Butt CM, Jones E. Achieving exceptional robustness for PFAS analysis in food with the next-generation SCIEX 7500+ system. SCIEX Technical Note. Available at: https://sciex.com/tech-notes/ food-beverage/food-and-beverage/achieving-exceptional-robustness-for-pfas-analysis-in-food-with-the-next-


generation-sciex-7500-plus-system (accessed August 15, 2025).


Mobile mass spectrometry system for residual gas analysis


Pfeiffer Vacuum+Fab Solutions, part of the Busch Group, has unveiled the HiCube Neo RGA - a fully integrated, mobile system for residual gas analysis that pairs vacuum pumping with high-sensitivity mass spectrometry.


At the heart of the system is the fusion of a HiCube Neo turbomolecular pump with the PrismaPro mass spectrometer, providing real-time gas composition analysis across a wide pressure spectrum - from atmospheric conditions down to high vacuum. Designed for use in environments such as coating systems, vacuum processing, and materials R&D, the HiCube Neo RGA supports both quality control and fault diagnostics.


Engineered with reliability in mind, the unit includes automatic filament shutdown when pressures exceed safe thresholds, protecting components and prolonging service intervals. Additional safety and contamination-control options include a built-in shut-off valve and recipient heater to desorb background gases that might skew results.


Aimed at ease of use and integration, the HiCube Neo RGA offers multiple I/O channels for sensor integration, a touchscreen interface, and PV MassSpec software for streamlined data access. The plug-and-play system is also available with a trolley mount, enabling quick relocation between test sites without disassembly.


Ideal for labs requiring flexible, high-precision gas monitoring, the HiCube Neo RGA represents a versatile tool for modern vacuum applications. More information online: ilmt.co/PL/5j7Z


64996pr@reply-direct.com


High-speed, compact FT-NIR spectrometer with Ethernet connectivity


Hamamatsu Photonics has unveiled the C16511-01, a powerful new addition to its spectrometer range. This compact FT-NIR spectrometer engine is currently the smallest of its kind on the market and is capable of capturing up to 275 spectra per second - delivering the speed and sensitivity needed for demanding industrial and research environments.


FT-NIR (Fourier Transform Near Infrared) spectroscopy is widely used in sectors such as pharmaceuticals, food production, agriculture, and chemical processing, where accurate and rapid analysis is essential for quality control and process monitoring. The C16511-01 is designed to make these high-performance capabilities more accessible, offering reliable spectral resolution and excellent light throughput in a highly integrated and cost-effective solution.


A key advantage of the C16511-01 is its spectral performance. Covering a response range from 1100 nm to 2500 nm, the device supports rapid spectral acquisition at speeds suitable for real-time process analytics. Its precision is underpinned by a built-in semiconductor laser, ensuring high wavelength accuracy across measurements.


Despite its impressive capabilities, the unit remains compact - measuring just 68 × 124 × 66 mm - making it easy to embed within existing OEM systems or portable instruments. The spectrometer supports diffuse reflection and transmittance modes, allowing users to analyse solids, liquids, or powders.


Connectivity is another standout feature. The Ethernet interface ensures straightforward integration with both Windows and Linux environments. Supplied evaluation software enables users to set measurement conditions, acquire and view data, while DLL specifications are openly provided for developing custom software applications.


This next-generation FT-NIR spectrometer is expected to be widely adopted across both industry and academia. Its speed and resolution make it particularly well suited for applications such as process analytical technology, material inspection and sorting, farm produce grading, pharmaceutical quality control, and plastic recycling operations.


More information online: ilmt.co/PL/kkjx 64788pr@reply-direct.com


INTERNATIONAL LABMATE - NOVEMBER 2025


Page 1  |  Page 2  |  Page 3  |  Page 4  |  Page 5  |  Page 6  |  Page 7  |  Page 8  |  Page 9  |  Page 10  |  Page 11  |  Page 12  |  Page 13  |  Page 14  |  Page 15  |  Page 16  |  Page 17  |  Page 18  |  Page 19  |  Page 20  |  Page 21  |  Page 22  |  Page 23  |  Page 24  |  Page 25  |  Page 26  |  Page 27  |  Page 28  |  Page 29  |  Page 30  |  Page 31  |  Page 32  |  Page 33  |  Page 34  |  Page 35  |  Page 36  |  Page 37  |  Page 38  |  Page 39  |  Page 40  |  Page 41  |  Page 42  |  Page 43  |  Page 44  |  Page 45  |  Page 46  |  Page 47  |  Page 48  |  Page 49  |  Page 50  |  Page 51  |  Page 52  |  Page 53  |  Page 54  |  Page 55  |  Page 56  |  Page 57  |  Page 58  |  Page 59  |  Page 60  |  Page 61  |  Page 62  |  Page 63  |  Page 64