Chromatography 9
User-friendly detector for molecular weight analysis
Testa Analytical’s BI-MwA Molecular Weight Analyzer offers a streamlined solution for labs and educational facilities seeking rapid, reliable determination of macromolecular weights without the complexity of a full GPC/SEC setup.
This pre-aligned, static light scattering detector is ready to use straight out
of the box, allowing users to generate accurate results with minimal training. Simply introduce your sample into the low-volume, 7-angle fl ow cell, and the high-sensitivity CCD detector captures scattered light automatically. The intuitive software then calculates the absolute molecular weight by extrapolating data to zero angle.
The BI-MwA’s innovative vertical fl ow path prevents trapped bubbles, a common challenge in multi-angle light scattering systems, while its conical fl ow cell design ensures complete fl ushing between samples. This eliminates contamination risks and keeps workfl ows clean and effi cient.
Ideal for labs processing a limited number of samples, the BI-MwA combines precision, simplicity, and reliability in a compact package.
More information online:
ilmt.co/PL/VGmy 65965pr@reply-direct.com
Columns for proven polar compound analysis
For over two decades, The Cogent Diamond Hydride™ TYPE-C™ HPLC Columns have been a trusted solution for scientists demanding precision, versatility, and consistency in their separations. These columns are built on a silica surface that ensures precise retention of polar compounds, making them ideal for analysing metabolites, amino acids, highly polar compounds as well as complex polar biological compounds found in plasma, urine, and saliva.
What truly distinguishes Diamond Hydride™ columns is their compatibility with Aqueous Normal Phase (ANP) chromatography, a powerful technique specifically suited for the retention and separation of highly polar compounds. By bridging the gap between reversed-phase and HILIC methods, ANP enables greater flexibility and efficiency in LCMS workflows—making these columns an ideal choice for metabolomics, pharmaceutical, and bioanalytical research.
Backed by Microsolv’s renowned technical support and comprehensive resources, method development and troubleshooting are streamlined and efficient.
Whether you’re working with amino acids, organic acids, carbohydrates, or peptides, Diamond Hydride™ TYPE-C™ Columns offer the selectivity, stability, and reliability that experienced chromatographers rely on.
Explore the full product line and discover how these columns can elevate your analytical capabilities: More information online:
ilmt.co/PL/nXKL and
ilmt.co/PL/37XB
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pr@intlabmate.com ADVERTORIAL High sensitivity purity analysis of peptide-oligonucleotide conjugates
Peptide-oligonucleotide conjugates (POCs) provide an improved intracellular delivery, leading to an enhanced therapeutical potential of therapeutic oligonucleotides. Most established synthesis workfl ows rely on postsynthetic coupling with separate preparation steps for oligonucleotides and peptides. Exact differentiation of structurally similar impurities remains technically demanding and requires analytical precision.
This Application Note presents an optimised IP-RP method for a model POC, providing a reliable foundation for quality control workflows. The model POC is using a 21mer DNA sequence conjugated to a nonaarginine peptide conjugated through a click reaction.
For an optimised method different common ion-pairing agents were screened. Butylammonium acetate provided the most promising results. Furthermore, the pH and gradient were optimised to a shallow gradient of 7 -17% acetonitrile in 10 minutes at pH 7. The highest resolution was obtained at an elevated temperature of 80°C.
A wide-pore YMC Accura Triart Bio C18 column (30 nm) was used for prior investigations, because this column is known for its suitability in oligonucleotide analysis. To assess potential alternatives, YMC Accura Triart Bio C4 (30 nm pore) and YMC Accura Triart C18 (12 nm pore) columns were also tested under the already optimised conditions. All columns were equipped with the bioinert YMC Accura coating to mitigate interactions with metal surfaces. This screening confi rms YMC Accura Triart Bio C18 as ideal column for the analysis of the POC (not shown).
Optimised conditions
Under optimised conditions using a wide pore YMC Accura Triart Bio C18 with a BAA/ acetonitrile eluent system, the method enables full separation of modified and unmodified oligonucleotides alongside the POC (Figure 1). All analytes elute with sharp peaks, delivering high resolution and analytical reliability suited for demanding quality control workflows.
Find all details of the method development in the Application Note.
More information online:
ilmt.co/PL/8eBp and
ilmt.co/PL/ZOmz
ACS Omega 2025, 10, 20, 20578-20584
65780pr@reply-direct.com
Figure 1: Analysis under optimised conditions of the crude POC (pink), the unmodifi ed oligonucleotide (brown) and the modifi ed oligonucleotide (orange) [1].
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