Practical Ultra-High Performance Liquid Chromatography (UHPLC) The potential to increase chromatographic efficiency and resolution along with significant savings in solvent cost
and analysis time have driven the uptake of UHPLC to many application areas. This discussion outlines how instrument and column technologies continually evolve to meet the requirements of UHPLC, providing new options for chromatographers. Example data are provided to show the high speed and high resolution options of UHPLC. Advanced topics such as HPLC to UHPLC translations using free downloadable tools are also covered.
Top: separation of six peptides on an ACE Excel 5 C18 HPLC column installed on an HPLC instrument. Bottom: the same separation translated to an ACE Excel 1.7 C18 UHPLC column installed on a VWR Hitachi ChromasterUltra Rs UHPLC system.
The method translation was achieved using the ACE LC Translator Tool. Mobile phase: A = 0.05% TFA in H2O, B = 0.05% TFA in MeCN. Temperature: 60 °C. Detection: UV, 220 nm. Sample: 1. Gly-Tyr, 2. Tyr-Tyr-Tyr, 3. Val-Tyr-Val, 4. Oxytocin, 5. Angiotensin II, 6. Leu-
enkephalin.
Intelligent Solutions for Method Development
• High efficiency UHLPC and HPLC columns • Ultra-inert for maximum performance and reproducibility
• Novel chemistries – including reversed-phase and HILIC – for optimum selectivity
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