6


to the three forms of the protein and have observed the subsequent changes in their aggregation rates. The group also identified several novel inhibitors of aggregation, along with a potential mechanism of their action.


These sessions were followed by a thirty-minute coffee break, which was held in the exhibition area and permitted time to meet with vendors.


The Wednesday morning two parallel sessions continued after the coffee break - before breaking for lunch at 13:00 with sessions on:


Session 3 - MS Solutions for Challenging Analytes


Chair: Dr Jackie Mosely (Durham University)


Dr Andy Ray (AstraZeneca) - Application of Selected Ion Flow Tube Mass Spectrometry (SIFT-MS) to the analysis of pharmaceutical products.


Dr Caroline Dessent - Shining Light into Mass Spectrometers: UV Laser-interfaced commercial mass spectrometers as tools for spectroscopic identification of isomeric molecular ions.


Kayleigh Arthur - Targeted and untargeted metabolic profiling by incorporating scanning FAIMS into LC-MS.


Edward Wilmot - Analysis of Gasoline using Atmospheric Pressure Ionisation Mass Spectrometry.


Of interest in these sessions were:


Dr Caroline Dessent (University of York) reported a series of results obtained using custom-adapted laser-interfaced commercial ion-trap mass spectrometers (Bruker Esquire and AmaZon). The results presented were from a UV photodissociation spectroscopy study of the gaseous structure of protonated nicotinamide (NA), produced using electrospray ionisation which demonstrated that by performing low- resolution UV spectroscopy within the commercial mass spectrometer, spectra can be acquired that allow different structural isomers of protonated NA to be distinguished (i.e. isomers associated with protonation at different sites). Photoabsorption and photofragment action spectra were obtained across the full spectral range scanned (215-350 nm), and reveal that gaseous protonated NA existed with either the pyridine or amide group protonated. Analysis of the photofragment action spectra indicated that that the ratio of pyridine/amide protonation is approximately 1.1:1. The results presented demonstrate the broad potential of such measurements as a relatively straightforward analytical tool for identifying geometric isomers that display UV chromophores.


Kevin Giles (Waters Corporation) discussed a brief history of RF ion guide development with an introduction to their operating principles and utilisation in mass spectrometry systems. The use of ion guides operating with radio frequency (RF) confining voltages is commonplace in current mass spectrometry systems and their use has transformed the performance and functionality of modern instruments. A more focussed perspective followed on the role of these guides operating in higher pressure regimes where they provide both efficient ion transportation and additional functionality. Recent developments in ion guide technology were highlighted, including ion mobility separators and advanced ion manipulation approaches.


After lunch, which included an optional Corporate Lunch Seminar sponsored by Thermo Fisher Scientific, the Wednesday afternoon from 15:00 was dedicated to the exhibition and time to peruse and discuss the over seventy posters which were presented by their author’s.


A drinks reception and the conference dinner rounded off a full first day of the BMSS Annual Meeting and Conference.


Thursday morning started with two parallel sessions.


Session 5 - New MS Technologies in Proteomics


Chair: Professor Claire Eyers (University of Liverpool)


Dr Richard Scheltema (Utrecht University) - Extending the Q Exactive Orbitrap with UVPD; application to peptides, proteins, and protein complexes.


Thomas Powell - Subcritical Water Processing of Proteins: Amino acid side-chain modifications.


XueXia Huang - Photo-affinity Labelling based Structure-Activity Relationship study of Hsp90 C-terminal Domain.


Federico Floris - 2D FT-ICR MS for Top-Down Proteomics.


Session 6 - Small Molecules Chair: Dr Susan Crosland (Syngenta)


Professor Perdita Barran (University of Manchester) - Structural Information to distinguish Small Molecules with Ion Mobility Mass Spectrometry (IM-MS)


Dr Patricia Wright - Predicting collision- induced dissociation (CID) fragmenation: understanding the role of the mobile proton small molecule fragmentation.


Florence Lai - Detection of DDT in small quantities of mosquito by thermal desorption GCGCMS.


Professor James McCullagh - Targeted and untargeted metabolic profiling of glioma cells expressing IDH1 mutations.


Session 4 - Fundamentals and Instrument Developments


Chair: Professor Gareth Brenton (Swansea University)


Dr Kevin Giles (Waters Corp) - The (not so) humble ion guide.


Dr Steve Bajic - An Aerodynamic Perspective on Impactor API Sources.


These sessions were followed by a thirty-minute coffee break, which was held in the exhibition area and permitted time to meet with vendors.


The pair of Thursday morning parallel sessions continued after the coffee break - before breaking for lunch at 12:30 with sessions on:


Session 7 - Emerging technologies for Processing MS Data


Chair: Dr Mark Barrow (University of Warwick)


Dr Robert Mistrik (HighChem Ltd) - mzCloud: A comprehensively annotated LC/MSn database for the identification of unknowns.


Dan Addison - Lessons from historical data: From data annotation to data mining and beyond, an evolving approach to MS data.


Professor Peter O’Connor - The next dimension in proteomics using two- dimensional mass spectrometry.


Dr Christopher Wootton - Supercharging of analytes via a novel modified Ion source.


Dr Pavel Ryumin - Liquid AP-MALDI enables rapid and sensitive analysis with multiply charged ions.


Edwin Aponte Angarita - Molecular dynamics simulation protocol for spectra assignment in ion mobility spectrometry.


Session 8 - Mass Spectrometry in Environmental and Forensic Analysis


Chair: Dr Simona Francese (Sheffield Hallam University)


Dr Melanie Bailey (University of Surrey) - Ion Beam and Mass Spectrometry Imaging in Forensic Science.


Professor Rainer Cramer - Evaluating milk adulteration and animal health using liquid AP-MALDI MS profiling.


Lisa Deininger - Out Damned Spot! - Bottom Up Proteomics for the Analysis of Bloodied Fingermarks.


Dr David Baker - Quantitative analysis of 646 pesticides (1,929 MRMs) using high-sensitivity LC-MS/MS with a fast 10.5 gradient.


A break of one hour for lunch, during which the BMSS Annual General Meeting took place, preceded the afternoon sessions, the BMSS Chairs Plenary Lecture and the closing farewell.


Session 9 - MS in Translational Medicine


Chair: Professor Mike Morris (Waters Corp)


Dr Kevin Mills (UCL) - Mass Spectrometry in the Clinic.


Dr Joanna Denbigh - Exploring the effect of a combined drug therapy on the lipidome of acute myeloid leukaemia cells using mass spectrometry-based lipidomics.


Dr Liam Heaney - Exploring the gut microbiome with mass spectrometry.


Richard Mbasu - Novel Targeted Strategies for Heart Failure Biomarkers.


Session 10 - Mass Spectrometry in Synthetic Biology


Chair: Professor Neil Oldham (University of Nottingham)


Dr Nik Rattray (University of Manchester) - The role of Mass Spectrometry in Driving the Design-Build-Test-Learn cycle.


Lucio Manzi - Mapping the interactions between the acyl carrier protein and a dehydratase domain installing a cis-double bond using carbene footprinting and mass spectrometry.


Ewa Szula - Metabolic analysis of small number of mammalian cells - development of mass spectrometry technologies.


Dr Chloe Martens - Deciphering the molecular mechanisms of sugar transport using a mass-spectrometry based methodology.


The final lecture the Plenary – BMSS Chair’s Lecture was given by Professor Jack Henion of Cornell University and Advion, Inc and was titled ‘What Will Mass Spectrometry Look Like in The Future?’.


Where he reflected on the last 50 years and gazed into his crystal ball and gave predictions for what the next 50 years will bring in regard to the ‘fit and form’ as well as the ease-of-use and analytical capabilities.


Late Thursday afternoon saw the presentation of various BMSS awards by the BMSS Chair Dr Christine Eckers as part of the closing ceremony.


The prizes that were awarded were as follows:


The Barber Prize, sponsored by LGC, was awarded to the best new and upcoming researcher’s oral presentation at the 2016 meeting. This year’s awardee was Lisa Deininger, Centre for Mass Spectrometry Imaging, Biomolecular Sciences Research Centre, Sheffield Hallam University for her oral presentation titled ‘Out Damned Spot! - Bottom Up Proteomics for the Analysis of Bloodied Fingermarks’ which discussed how the currently employed enhancement and detection techniques for blood and blood in fingermarks are not confirmatory due to targeting generic compound classes like proteins, and as such are not sufficiently specific and prone to false positives. She continued to describe how the work presented previously has been developed further to devise a protocol for proteomic in situ analysis of bloodied fingermarks with MALDI-MS imaging, enabling the mapping of blood peptides to fingermark ridges and thus establishing a strong link between the suspect and the cause of bloodshed. In order to achieve this, it was necessary to increase the trypsin concentration significantly compared to on tissue digests.


The Bordoli Prize, sponsored by Waters, is awarded to the best new and upcoming researcher’s poster presentation at the 2015 meeting. This year’s awardee was Patrick Knight from the Astbury Centre for Structural Molecular Biology, University of Leeds for his poster titled ‘Characterising the interaction of ataxin-3 and the poly-glutamine aggregation inhibitor QBP1’ which described how given the current growing social and economic burden of amyloid neurodegenerative disorders (Alzheimer’s) there is an urgent need to increase the understanding of amyloid diseases in order to develop effective therapies. To this end nESI-ToF-MS was used to directly


LAB ASIA - NOVEMBER/DECEMBER 2016


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