LIFE SCIENCES
capture a large field of view at high resolutions. CMOS chips are larger and have higher pixel counts of between four and six megapixels, as opposed to EMCCD sensors that have less than one megapixel resolution. Having a large field-of-view and the
capability to zoom in retrospectively is of particular interest to scientists working in fluorescence microscopy. In contrast to the common
misunderstanding that higher magnifications require higher camera resolution, higher resolution is, in fact, only effective when working at low magnifications. Giacobone added, ‘when using a 60X or 100X oil objective, for example, a one-megapixel camera will normally be sufficient; a higher pixel count will just increase data volume without providing any extra information. However, when using lower magnifications, higher pixel counts are required to capture more detail, allowing the user to zoom in aſterwards.’ For this reason, as Giacobone explained: ‘We have an increasing number of customers who no longer use 60X or 100X objectives in fluorescence imaging, instead wanting to use maybe a 20X or a 10X
High resolution is crucial for documentation purposes. At low magnification, retrospective analysis is only possible with high resolution.
objective.’ When wanting to correctly capture all of the optical resolution, the required megapixel count rapidly increases as the magnification decreases. Simply put, if you want to capture a bigger area containing a lot of detail, you need a higher number of pixels, and finer pixels, to capture those details. Having a larger field-of-view with a higher
resolution not only provides the scientist with more information, but increases the speed of imaging. One method used to create an image of a larger sample area is to scan smaller areas of the sample, and then stitch the images together to form a large picture which the user
can later zoom in on. Giacobone explained: ‘Tink of Google maps − it is basically a collection of small images that you can pan around and zoom in on.’ By having a larger sensor, at each scanning step the camera can capture a larger section of the sample, meaning that fewer scanning steps are involved, and the sample can be scanned more quickly. ‘Te industry is shiſting towards bigger chip
sizes,’ Giacobone added. ‘Te trend is towards having a one inch sensor or more in every type of camera, either colour or monochrome. Interestingly, the evolution in this area mimics what is happening in the consumer market.’
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