Applications of the bioreactor system
Monitoring of cell cultures The automated ambr micro bioreactor system overcomes the drawbacks surrounding the simultaneous culturing of large numbers of cell line variants. With up to 24 or 48 miniatur- ized bioreactors containing 10–15 mL of media (Figure 1), ambr enables the successful small- scale production of a large number of cultures, with less effort and potential for error. With active cooling, the culture can be set and maintained at a temperature of 27 o
C, which is important for the
successful infection and maintenance of insect cell lines.
Automated liquid handling The ambr workstation and flexible software enable automated liquid handling for media filling, inoculation, reagent or feed addition, cell sampling, splitting, and virus infection. Alongside monitoring and controlling culture conditions, the system eliminates the daily manual tasks of culture maintenance and sample tracking. With the ability to add nutri- ents, reagents, or cells without disturbing the continual movement of the impellers stirring the culture, minimal disruption of cell growth is achieved.
The workstation operates inside a biosafety cabinet, providing a reliable platform for sterile liquid handling such as feeding and sampling. Automated monitoring of dissolved oxygen and pH level through sensor spots found on the ambr bioreactors is also beneficial to cell growth. Active control of these two culture parameters inside each individual bioreactor provides the potential to optimize and enhance culture growth.
Bioreactor system software
and footprint In addition to providing continual culture monitoring, the system’s software can enable reports to be processed and transmitted to e- mail for remote monitoring at any time of the day or night. The ambr workstation allows con- trol of feeding, sampling, and maintenance of environmental conditions for each individual bioreactor, providing the ability to study the effect of a range of conditions on a cell line
The Flow SolutionTM 3100 improves
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or a range of recombinant virus clones. With up to 24 or 48 bioreactors per workstation, the system also provides the ability to include more control replicates for experimental and quality control.
With a small footprint—the size of a laboratory bench—the ambr workstation significantly re- duces the amount of laboratory space required. With lower volumes, it also significantly reduces media and reagent costs.
Proof of concept of automation for high-throughput
multiprotein expression In a recent collaborative study carried out by TAP Biosystems and EMBL,5
two insect cell
lines (Sf21 and Hi5) were cultured in triplicate in GIBCO (Life Technologies, Carlsbad, CA) and Hyclone Medium (Thermo Fisher Scientific, Waltham, MA) in the ambr system. The results
AUTOMATING WET CHEMISTRY IMPROVES LAB PRODUCTIVITY
AMERICAN LABORATORY • 33 • SEPTEMBER 2013
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