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slowly compared to bare GCE and higher current densities were observed for o-phenylenediamine oxidation, confi rming the higher electroactive area in MGCE. No notable diff erences were observed in the cyclic voltammogram with and without the presence of UA (not shown), which demonstrates that UA does not have any electroactivity on the MGCE under the chosen experi- mental conditions. Further, the structure of the template was not changed during the polymer synthesis around UA.


Electrochemical characterization of MIP-MGCE and nonmolecularly


imprinted MIP (nMIP)-MGCE As shown in Figure 2a, a pair of typical redox peaks of K3


[Fe(CN)6 ] appeared


at bare GCE (curve a). When the surface was covered with the MWNTs– COOH layer, an increment of the redox peak current was observed (curve b). It is well known that MWCNTs–COOH have small dimensional size and excellent catalytic activity, which facilitated the redox reaction of Fe(CN)6


3–/4– at the MGCE.


When the imprinted fi lm was electrosynthesized on the surface of the MGCE, the peak current was not observed (curve c). It is possible that the K3


[Fe(CN)6 3− increased. This can be ascribed to the fact


that once the template was removed, the formation of recognition sites or binding cavity made electronic transmission possible again.


] could not pass through the layer of polymer to arrive at the


surface of the electrode. As shown in curve d, after the template removal, the redox current of Fe(CN)6


Figure 3 – Molecular structures of uric acid (UA), caff eine (CA), theophyl- line (TP), adenine (AD), xanthine (XA), hypoxanthine (HXA), ascorbic acid (AA), and dopamine (DA).


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