22 JAAHA /
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RETROSPECTIVE STUDY Clinicopathologic Significance of Histologic Grade, Pgp, and P53 Expression in Canine Lymphoma
RS Dhaliwal, E Kitchell, EJ Ehrhart, VE Valli, NG Dervisis
To characterize the expression of P-glycoprotein (Pgp) and p53 in different histologic grades of canine multi- centric lymphosarcoma (LSA), 31 cases of LSA without prior treatment were studied. The expression levels of the Pgp and p53 proteins were evaluated for their clinicopathologic significance among standard histo- logic evaluation. Immunohistochemistry (IHC) was per- formed on formalin-fixed, paraffin-embedded archival samples of 31 previously untreated LSA cases to detect the expression of Pgp and p53. All dogs were subse- quently treated with a combination chemotherapy protocol. Remission and survival durations were evalu- ated for correlation with histologic grade and presence of drug resistance markers. Of the 31 cases, 24 (80%) and 7 (22%) were positive for Pgp and p53, respectively. Overall, the median survival and duration of remission in the study was 246 days and 137 days, respectively. The National Cancer Institute working formulation his- tologic grade was not associated with either survival or duration of first remission (DOR). The Pgp protein expression and DOR and survival was not statistically significant. Expression of p53 was statistically correlated with survival.
RETROSPECTIVE STUDY Efficacy of IG for Prevention of GDV in Dogs
ME Benitez, CW Schmiedt, MG Radlinsky, KK Cornell
Incisional gastropexy (IG) is routinely performed as ei- ther a prophylactic procedure to prevent occurrence of gastric dilatation-volvulus (GDV) or at the time of surgi- cal correction of GDV to prevent recurrence. Despite its common use, the long-term efficacy of the IG procedure has not been reported. The hypothesis of this study was that IG performed either during surgical treatment of GDV or as a prophylactic measure would effectively prevent GDV. Medical records of 61 dogs undergoing IG following either gastric derotation for treatment of GDV or as a prophylactic procedure were evaluated retrospectively. Median follow-up time for all dogs was 717 days (range, 49–2,511 days). Of the 61 dogs, 27 had prophylactic IG performed. The remaining 34 dogs pre- sented for GDV and had an IG performed during surgi- cal treatment of GDV. No dog experienced GDV after IG. Recurrence of gastric dilatation (GD) alone was noted in 3 of 34 patients (8.8%) undergoing IG during surgery for GDV and in 3 of 27 patients (11.1%) treated prophylacti- cally with IG. This study confirmed the efficacy of IG for the long-term prevention of GDV in dogs.
RETROSPECTIVE STUDY Accuracy of US-Guided FNA of Focal Liver Lesions in Dogs: 140 Cases (2005–2008)
KL Bahr, LC Sharkey, T Murakami, DA Feeney
Medical records from dogs having abdominal ultrasound (US) performed between March 2005 and October 2008 were reviewed for detection of focal liver lesions (FLL) with both cytologic and histologic sampling. Samples were classified as to either the presence or absence of major categories of pathologic processes, including ma- lignant neoplasia, inflammation, hyperplasia/benign neoplasia, vacuolar change, extramedullary hemato- poeisis, cholestasis, necrosis, and no microscopic abnor- malities. Evaluation of selection bias was performed by review of the relative distribution of cytologic diagnoses for cases with histology compared with cases excluded from the comparison analysis because histology results were not available. Cytology had the highest sensitiv- ity for vacuolar change (57.9%), followed by neoplasia (52.0%). Cytology had the highest positive predictive value (PPV) for neoplasia (86.7%) followed by vacu- olar change (51.6%). Cytology had lower sensitivity and PPVs for inflammation, necrosis, and hyperplasia. The ability of cytology to characterize disease in canine FLL varies by pathologic process. Clinicians can have a high degree of confidence when a cytologic diagnosis of neoplasia is given; however, cytology is less reliable for excluding the potential for neoplasia. Cytology has a low sensitivity and PPV for inflammation and a limited diag- nostic performance for the diagnosis of vacuolar change.
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jaaha.org for more information. Trends magazine, May 2013
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