Microscopy & Microtechniques 51 Optical Sectioning Device for High-Contrast and Blur-Free Imaging


Olympus announces the latest addition to its product lineup - the SILA Optical Sectioning Device. Designed to empower researchers and professionals in obtaining high-contrast images from deep within their samples, this cutting-edge device revolutionises optical sectioning capabilities.


The SILA optical sectioning device combines speckle illumination and HiLo microscopy techniques to deliver exceptional imaging results. By capturing two illuminated images and employing advanced mathematical processing algorithms, out-of-focus light is effectively removed, resulting in high- contrast images. What sets this technology apart is its user-friendly nature, requiring no specialised calibration and making it accessible to users at all skill levels.


SLIDEVIEW™ VS200 v. 4.1 software seamlessly integrates with the SILA optical sectioning device, enabling users to effortlessly acquire optical sectioning images. Regardless of imaging depth, the software ensures consistent optical sectioning capabilities, allowing users to capture stunning images even from deep within their samples.


One of the key advantages of the SILA optical sectioning device is its versatility and compatibility with existing VS200 systems, including those equipped with a slide loader. Its compact design allows for easy installation onto the scanner’s fl uorescence illuminator. Moreover, the device is compatible with a wide range of sample types, including cleared and fi xed cells and tissues thicker than 100 microns, accommodating various magnifi cation requirements.


Conventional detection methods often fall short in identifying transparent and morphologic features on samples, potentially missing critical targets. However, SLIDEVIEW v. 4.1 software now incorporates TruAI™ technology, which excels at accurately segmenting these features and distinguishing them from visually similar structures.


To streamline data management and reduce the data burden, TruAI leverages its selective scanning mode, intelligently identifying the sample area and skipping irrelevant sections. This optimisation of data handling encompasses storage, image uploading, and sharing, enhancing effi ciency throughout the workfl ow.


Users can seamlessly upload their images to the database, effortlessly differentiate between users, and leverage offl ine visualisation and annotation tools for enhanced analysis and collaboration. More information online: ilmt.co/PL/mVRB


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CE-IVD Certifi ed Digital Live Microscope for Clinical Use


German digital microscopy company PreciPoint has announced the launch of its iO:M8 Digital Live Microscope, which recently received CE-IVD certifi cation under the new IVDR regulation. The microscope is designed to digitise intra-operative examinations during cancer surgery and provides high-resolution, real-time images of samples. The iO:M8 can accommodate up to four slides simultaneously, making it easy to use and providing pathologists with more information about the tissue removed during surgery. PreciPoint has received numerous awards and recognition, including Top Employer 2023 and Top100 Innovator 2022, for its bespoke solutions to bridge the gap between digital workfl ows and pathologists.


More information online: ilmt.co/PL/WM7n 60111pr@reply-direct.com


New FLIM Functionality for Enhanced Raman Microscopy


Renishaw has collaborated with Becker & Hickl GmbH, specialists in time-correlated single photon counting (TCSPC), to integrate fl uorescence lifetime imaging microscopy (FLIM) functionality into its inVia™ confocal Raman microscope. This combination of Raman and FLIM in a single instrument provides pixel-to-pixel correlation between FLIM and Raman images, enhancing sample analysis across a wide range of applications.


The integrated system utilises a pulsed laser to interact with a fl uorophore, and measures the fl uorescence lifetime with the aid of an advanced detector and high-speed electronics. This lifetime refers to the duration that a fl uorophore remains in an excited electronic state, emitting a photon as it returns to the ground state. By analyzing the decay of fl uorescence, it is possible to observe changes in molecular conformation due to its sensitivity to the molecular environment. The FLIM technique has the versatility to explore various properties such as pH/ion/oxygen concentration and molecular binding.


FLIM and Raman microscopy are two complementary label-free imaging methods used in the analysis of biological cells and tissue sections. FLIM enables the visualisation of molecular interactions, and its imaging speed is at least 10 times faster than that of Raman imaging. Raman spectroscopy provides high chemical specifi city and high-resolution chemical images. Combining these techniques allows for the rapid identifi cation of regions of interest using FLIM, followed by detailed chemical and structural analysis using Raman imaging.


The FLIM data analysis is supported by the Becker & Hickl software, which offers comprehensive features such as multi-exponential, incomplete, and shifted-component analysis models. Additionally, the software includes a proprietary phasor analysis tool that can differentiate between lifetime populations. The Renishaw MS30 high-speed encoded stage is employed for both imaging modalities, enabling direct overlay of fl uorescence lifetime and Raman images with high accuracy.


Renishaw Spectroscopy Business Development Manager, David Reece, commented: “We are pleased to announce a further enhancement to our award-winning inVia Raman microscope. Our collaboration with the leaders in FLIM technology, Becker and Hickl, has produced a combined FLIM Raman microscope leading to exciting new application areas.”


More information online: ilmt.co/PL/GbzW and ilmt.co/PL/D358 60110pr@reply-direct.com New Software Suite Enhances Raman Microscopy Capabilities


WITec GmbH, a specialist in Raman microscopy, has launched Suite SIX, the latest software for controlling Raman and correlative measurements, as well as data acquisition and post-processing. The software has been designed to further enhance the highly confi gurable alpha300 microscope series by providing multiple user profi les, support for regulatory compliance, and advanced sample navigation features.


Suite SIX’s multi-user management feature empowers the site administrator to assign instrument functionalities and data access rights to each system login, assisting labs in their efforts to comply with good practice (GxP) guidelines such as the US Food and Drug Administration’s 21 CFR Part 11, which is essential for pharmaceutical research and development. Moreover, the software enables users to create a repository of unique hardware confi gurations, which simplifi es the process of conducting recurring experiments.


As described by Dr Wolfram Ibach, Director of Software at WITec: “Suite SIX lets industrial facilities and research groups virtually customise the microscope for each individual user, with the appropriate feature set and data handling protocols. This accelerates the experimental workfl ow while ensuring compatibility with their training and standard operating procedures.”


TrueOrigin, the portable coordinate system integrated within Suite SIX, enables quick location of measurement areas relative to markers on the sample holder or features on the sample. This accelerates the correlation of data collected through multiple techniques from the same position, even if the sample is transferred between instruments. It also allows users to import sample images captured with an external camera to navigate to a specifi c area of interest and facilitate project documentation.


“Anyone performing correlative measurements on multiple instruments knows how time-consuming it can be to fi nd a particular area or feature after re-mounting a sample,” said Dr Thomas Dieing, WITec Product Manager. “Our portable coordinate system automates that process so researchers can go right to the next measurement, even on another microscope.”


WITec Suite SIX is available now. More information online: ilmt.co/PL/OVVB


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