50 Digital Microscope Simplifi es Cell Culture Checks


Mateo TL, a new digital transmitted light inverted microscope for routine cell culture, has been launched by Leica Microsystems. With Mateo TL, lab members can easily and comfortably check and document cell growth. By consistently measuring confl uency, they can be confi dent in the readiness of their samples for downstream experiments.


“Good cell culture sample preparation is an important starting point for every successful experiment, and now it has become even easier and more reliable with Mateo TL,” said James O’Brien, Vice President of Life Sciences and Applied Microscopy at Leica Microsystems.


“During the sample preparation process, subjective judgements are often used for sample readiness assessments, which can compromise the quality and the outcome of experiments, said Jennifer Kulhei, Product Manager, Life Science and Research Microscopy at Leica Microsystems.


With Mateo TL, labs can establish a reliable criterion for evaluating confl uency, improving experiment reproducibility. The confl uency algorithm measures the percentage of cell coverage in the image, enabling consistent measurements across multiple users. Additionally, users can work in a sterile environment to minimise contamination risk by placing Mateo TL directly under the fl ow hood, ensuring the integrity of their experimental outcomes.


Mateo TL is a user-friendly microscope that is easy to set up and use, even for non-experts. It is designed to support cell culture checks for a range of users, from principal investigators and postdoctoral researchers to PhD and master’s students, as well as lab technicians, all of whom may have varying levels of microscopy expertise. The microscope can be set up in less than a minute, providing a ready-to-use system that team members can start working on immediately. Once set up, cell culture checks are made easy for the whole team with Mateo TL’s user-friendly software interface, enabling simple live viewing and image acquisition.


In addition to its user-friendly design, Mateo TL streamlines cell culture checks by improving effi ciency and convenience. With its convenient fi le sharing capabilities, smart light and camera settings, and enhanced ergonomics, Mateo TL empowers teams to spend less time on routine cell culture checks and more time on their experiments.


More information online: ilmt.co/PL/v1B2 59953pr@reply-direct.com


All-in-One Cell Imaging System


Zeiss has launched its latest all-in-one cell imaging system, the Zeiss Axiovert 5 digital, which employs artifi cial intelligence (AI) and automatic functions to streamline daily tasks in the cell lab. This system enhances processes, from basic research to scientifi c routine, by enabling more effi cient and reproducible phase contrast and multichannel fl uorescence imaging. Additionally, the system is designed with an intuitive operating concept, allowing even inexperienced users to produce excellent images with ease. The settings and adjustments are carried out automatically, further simplifying the imaging process.


Laboratory technicians and researchers can employ brightfi eld, phase, and fl uorescence contrast techniques to examine cell or tissue cultures. With the press of a button, the system takes control and automatically modifi es the exposure time, captures the image, switches the channel, and restarts the process. The images are saved automatically, along with all the relevant metadata and scaling information of the microscope.


The Zeiss Axiovert 5 digital system utilises artifi cial intelligence to optimise and streamline workfl ows. By using the readily available AI modules, cell counting and cell confl uency can be automatically determined with a single click of a button. This allows users to quickly measure and display the percentage of cell coverage or number of cells in their Petri dish.


The ergonomic layout of the Zeiss Axiovert 5 digital is tailored to support appropriate system operation, making it an ideal fi t for multi-user environments. Users can easily trigger single snaps, multi-channel image acquisitions, or video recordings with just the press of a button, requiring minimal training or prior knowledge.


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Glass Bottom µ-Plates Enable High Throughput and Super-Resolution Microscopy


Microscopists now have high-throughput possibilities for super-resolution and TIRF thanks to Ibidi’s new glass bottom µ-plates. When using the µ-Plate 96 Well or 384 Well Black Glass Bottom, the samples are observed through a thin #1.5H D 263 M Schott glass coverslip with a thickness of 170 µm +/- 5 µm. Due to its superior optical quality, the µ-Plates are not only suitable for standard microscopy applications, but also ideal for total internal refl ection fl uorescence (TIRF), super-resolution microscopy (STED, SIM, (F)PALM, (d)STORM), and fl uorescence correlation spectroscopy (FCS).


When using the µ-Plate 96 Well or 384 Well Black Glass Bottom, the samples are observed through a thin #1.5H D 263 M Schott glass coverslip with a thickness of 170 µm +/- 5 µm. Due to its superior optical quality, the µ-Plates are not only suitable for standard microscopy applications, but also ideal for total internal refl ection fl uorescence (TIRF), super-resolution microscopy (STED, SIM, (F) PALM, (d)STORM), and fl uorescence correlation spectroscopy (FCS).


The µ-Plates have square wells and feature optimal fl atness properties, which are crucial for high-resolution microscopy in screening applications. The glass coverslip provides high accuracy and facilitates imaging from well-to-well across the entire plate. Additionally, the black walls ensure low well-to-well crosstalk in fl uorescence microscopy.


The µ-Plates are ideal for the cultivation, high throughput screening (HTS), and high-resolution microscopy of cells. They meet the requirements of the ANSI/SLAS standard, and are therefore compatible with a variety of imaging systems, pipetting robots, and plate readers.


Researchers who would like to test the new µ-Plate 96 Well or 384 Well Black Glass Bottom with their own experiments can get free samples. More information online: ilmt.co/PL/BKd4 59668pr@reply-direct.com Using Fluorescence Imaging for Improved Outcomes in Skin Cancer Surgery


University of Rochester Professor Michael Giacomelli recently received NIH funding for his project, ‘Fluorescence microscopy for evaluation of Mohs surgical margins’. His research objective is to enable real-time evaluation of pathology in skin tissue with an order of magnitude reduction in processing time as compared to frozen sections. The most common forms of cancer worldwide are basal cell carcinoma and squamous cell carcinoma. Mohs surgery is a widely used technique for the treatment of nonmelanoma skin cancer that obtains extremely low recurrence rates by imaging tissue as it is removed from the body to ensure complete resection. Mohs Micrographic Surgery, also called Mohs Surgery, is a specialised technique to remove non-melanoma skin cancers.


Nonmelanoma skin cancer (NMSC) is primarily diagnosed by histologic analysis of skin biopsy specimens in kerosene sections, which takes days to weeks before a formal diagnosis is made. Two- photon fl uorescence microscopy (TPFM) has the potential for point-of-care diagnosis of NMSC and other dermatologic conditions, which could allow diagnosis and treatment at the same site.


Professor Giacomelli developed a way to image multiple depth slices without frozen section processing‚ by implementing two-photon imaging, widely used in the neurosciences to noninvasively image slices at different depths in living brains. The lab team’s research has adapted these fl uorescence imaging technologies with rapid tissue labelling and image processing technologies. This enables real-time assessment of pathology in skin tissue. processing time compared to frozen sections has been reduced by an order of magnitude. The data collection time is further reduced using the lower- noised silicon photomultiplier detectors developed by Giacomelli’s group.


Toptica Photonics’ femtosecond laser system - FemtoFiber ultra 920 - chosen for the next iteration of the Giacomelli lab’s microscope is a possible solution for applications in non-linear microscopy like two-photon excitation of fl uorescent proteins and SHG based contrast mechanisms. With an emission wavelength of 920 nm, it provides the highest peak power for imaging with green and yellow fl uorescent protein markers (GFP, YFP) commonly used in pathology, neurosciences and other laser-related biophotonic disciplines. Researchers appreciate the usability of the system since it is both, maintenance free and very compact.


More information online: ilmt.co/PL/WMKQ 59545pr@reply-direct.com


INTERNATIONAL LABMATE - JULY 2023


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