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February / March 2012
Sub-2 µm Protein Analysis Biocolumns with C3 and Diphenyl Phases for Broader Selectivity and Better Peak Shape Released
Agilent Technologies announce it has expanded its family of sub-2 µm particle 300 Angstrom pore columns for reversed-phase liquid chromatography with rapid-resolution, high-definition ZORBAX 300SB-C3 and 300-Diphenyl 1.8 µm columns.
The columns extend the benefits of reversed-phase biomolecule separations to ultra-high-performance liquid chromatography (UHPLC). The C3 phase broadens the selectivity and improves peak shape and
recovery options for larger proteins, including antibodies. The diphenyl phase offers additional selectivity through pi-pi interactions with aromatic amino acids in the primary structure.
Agilent now offers the broadest range of sub-2 µm, wide-pore biocolumns for reversed-phase liquid chromatography,” said Agilent Product Manager Linda Lloyd. “The new 1.8 µm columns add to the scalability of the ZORBAX family of C18, C8 and C3 phases already available with 3.5 and 5 µm particles. We’re pleased to bring this higher order characterization and increased speed to UHPLC users.”
The 1.8 µm particle size and 300 Angstrom pore size bring UHPLC efficiency, resolution and robust quantitation to reversed-phase protein separations. Additionally, the columns are stable up to 1200 bar. Agilent’s proven StableBond technology for the C18, C8 and C3 columns, and the end-capped diphenyl with Pursuit chemistry, deliver symmetrical peaks – even at low pH – when using trifluoroacetic or formic acid mobile phase modifiers. Column life is not compromised.
The full ZORBAX rapid-resolution, high-definition family now includes 13 phases for small molecule applications, including HILIC, and four phases for large molecule separations. This broad range of options enables chromatographers to optimize their UHPLC separations with selectivity refinements. In addition, RRHD’s 1200 bar stability provides increased flexibility with flow rates and mobile phase selection.
For more information, visit
www.agilent.com/chem/biohplcproteins
Lightweight Compact Digital Liquid Flow Meter for Any HPLC Pump
Featuring a single chip microcontroller and accurate to ± 1% of calibrated flow rates the lightweight 12-ounce FlowCal 5000 HPLC liquid flowmeter available from Tovatech supports FDA compliance for IQ, OQ and PQ validations on any HPLC pump.
According to Tovatech Director, Dr Robert Sandor: "The FlowCal 5000 liquid flow meter is faster and more accurate than using timers while manually collecting fluid for gravimetric or volumetric measurement in calibrating HPLC pump flow rates. Flows from 0.05 to 25 millilitres per minute are independent of liquid type up to a 10 centipoise viscosity. Rates rounded to four digits are displayed on a backlit LCD while complete data rounded to a full 6-digit reading are output via a built- in RS232 interface."
The FlowCal 5000 liquid flowmeter is shipped with a UKAS traceable calibration certificate at 1.0 millilitre per minute or customers can specify standard 3-point calibrations of 0.5, 1.0 and 2.0 millilitres per minute, Dr Sandor said. He added: "On request we will calibrate the HPLC flow meter at user-specified flow rates within the unit"s 0.05 to 25 millilitres per minute operating range and provide FDA required annual re-certifications."
The HPLC liquid flow meter is designed for use at the end of the HPLC flow system. The package includes a mounting kit for freestanding (table), wall or stack mounting positions, or for mounting on standard wide-necked reagent bottles. Included is a carrying case and cleaning kit. An optional thermal printer is available. All wetted parts are made of PTFE, PEEK, DAI-EL, PERFLOUR or Pyrex to handle common HPLC solvents.
For more information please visit
www.tovatech.com
Restek Gains ISO Guide 34 and 17025 Accreditations On a Full Line of Certified Reference Materials
Restek’s reference standard manufacturing and QA testing laboratories in the USA have added ISO Guide 34 and 17025 accreditations to the company’s existing ISO 9001 registration. Issued by the American Association for Laboratory Accreditation (A2LA), these accreditationsserve as recognition that Restek and its laboratories meet the world-class quality standards established by the International Organization for Standardization (ISO).
In addition to catalogue listed items the new accreditation applies to Restek’s custom reference standards too.
Using Restek as your supplier for standards will enable you to: Satisfy the latest UKAS regulatory requirements, Benefit from the exceptional product quality and customer service needed to meet strict ISO 9001, Guide 34, and 17025 guidelines, Get the same reliability and documentation with custom-formulated solutions as you do with stock standards since both fall under Restek’s accreditation, Eliminate multiple orders by ordering primary- and secondary-source reference standards, GC and LC columns, sample prep supplies, and accessories from one supplier and instant on-line access to documentation via website
Restek products are available in the UK through Thames Restek UK Ltd, for more information
www.thamesrestek.co.uk
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