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these compounds pose, it is perhaps a relief to those who must perform characterisation and quality monitoring that there are robust column technologies available that afford both rapid and high resolution separations of a vast range of oligonucleotides, including single and double stranded DNA, those labelled with fluorescent dye, and failure sequences.
Here we have demonstrated modern and innovative chromatographic strategies for the analysis of this class of compounds for development and quality control purposes.
References
1. Offord, C., Oligonucleotide Therapeutics Near Approval, The Scientist, December 2016
http://www.the-scientist.com/?articles. view/articleNo/47499/title/Oligonucleotide- Therapeutics-Near-Approval/
2. Baek, J., Thayer, J., Wang, H., Birznieks, I., Liu, X., High Resolution LC/MS Analysis of Therapeutic Oligonucleotides on a New Porous Polymer-Based Reversed Phase Column, Poster:
https://tools.thermofisher. com/content/sfs/posters/PN-21515-LC-MS- Therapeutic-Oligonucleotides-WCBP2016- PN21515-EN.pdf
3. Dias, N.; Stein, C.A. Antisense Oligonucleotides: Basic Concepts
Innovative HILIC Columns for HPLC and UHPLC
Hilicon is a leading company in developing and manufacturing chromatography products for HILIC separation of polar compounds. It has recently introduced a broad range of HILIC columns under the brand names of iHILIC® Fusion(+), and iHILIC®
-Fusion, iHILIC® -
-Fusion(P). These columns can be used in the application areas, such as, ‘Omics’ research, pharmaceutical discovery, food and beverage industry, clinical diagnostics, and environmental monitoring.
The packed stationary phases are charge modulated amide silica or polymer particles that are covalently bonded with neutral, positively charged, and negatively charged hydrophilic functional groups, following Hilicon’s longtime experience in HILIC technology. Therefore, iHILIC columns provide tailor-made and complementary selectivity, excellent durability, and ultra-low bleeding, which improve the productivity and quality of chemical and biological analysis.
Beside the HILIC columns, Hilicon also provide HILIC type SPE cartridges (iSPE® -HILIC) for solid phase extraction and purification
of polar compounds from various sample matrix. The purification of glycan/glycopeptides or toxins are two of the most important applications. For further information email
info@hilicon.com or visit
www.hilicon.com.
and Mechanisms, Molecular Cancer Therapeutics, 2002, 1, 347-355.
4. Resnier, P.; Montier, T.; Mathieu, V.; Benoit, J.P.; Passirani, C. A review of the current status of siRNA nanomedicines in the treatment of cancer, Biomaterials, 2013, 34, 6429-6443
5. Jose V. Bonilla, G. Susan Srivatsa, Handbook of analysis of oligonucleotides and related products, p10, 2011 CRC Press
6. Baek, J., Thayer, J., Lin, S., Liu, X., Separation of Large Double-Stranded DNA (dsDNA) Fragments Using a Wide-Pore Reversed-Phase Chromatography Column, Application note:
https://tools.thermofisher. com/content/sfs/brochures/AN-21533-LC-
NEW ANALYTICAL PYROLYSIS SYSTEM
THERMAL SAMPLE INTRODUCTION SYSTEMS FOR GC
Pyrolysis, EGA studies, TD, and headspace with GCMS polymer/additive libraries High pressure pyrolysis with reactant gas/steam for pre-pilot reactor studies Analysis of solids and liquids with ten step thermal analysis to 1400°C Automated TD/pyrolysis multistep analysis of up to 36 samples
TEL: 0844 800 4220 OUTSIDE UK: +44 (0) 191 519 4504 EMAIL:
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