search.noResults

search.searching

dataCollection.invalidEmail
note.createNoteMessage

search.noResults

search.searching

orderForm.title

orderForm.productCode
orderForm.description
orderForm.quantity
orderForm.itemPrice
orderForm.price
orderForm.totalPrice
orderForm.deliveryDetails.billingAddress
orderForm.deliveryDetails.deliveryAddress
orderForm.noItems
Understanding Magnifi cation


Table 5 : Object fi eld (OF) data (Equation 13) for an image from a digital microscope with 5 MP image sensor (DMS1000) and stereo microscope (M205 A) equipped with a 5 MP digital camera (MC170 HD) showing the range from minimum to maximum values. The digital and stereo microscope discussed in this article are used often for inspection, such as during manufacturing. Generally, their maximum magnifi cation values are lower than compound or higher performance digital microscopes.


Digital microscope with 5 MP sensor


M TOT PROJ


0.08:1 (0.08×) 3.96:1 (3.96×)


Stereo microscope with 5 MP camera


M TOT PROJ


0.16:1 (0.16×) 32:1 (32×)


Object field (OF) of Sensor


w (mm) 77


1.5 h (mm)


58.1 max OF 1.2


min OF


Object field (OF) of Sensor


w (mm) 39.1 0.19


h (mm)


29.5 max OF 0.14


min OF


[6] C Hoyer, Chair Technical Committee , ISO 10934-2: 2007: Optics and optical instruments -- Vocabulary for microscopy -- Part 2: Advanced techniques in light microscopy , International Organization for Standardization (ISO) , Geneva, Switzerland , 2007 , www.iso.org/standard/ 39473.html .


[7] C Hoyer, Chair Technical Committee , ISO 19012-1:2013: Microscopes -- Designation of microscope objectives -- Part 1: Flatness of field/Plan , International Organization for Standardization (ISO) , Geneva, Switzerland , 2013 , www.iso. org/standard/61652.html .


[8] C Hoyer, Chair Technical Committee , ISO 19012-2:2013: Microscopes -- Designation of microscope objectives -- Part 2: Chromatic correction , International Organization for Standardization (ISO) , Geneva, Switzerland , 2013 , www.iso. org/standard/61836.html .


[9] C Hoyer, Chair Technical Committee , ISO 18221:2016: Microscopes -- Microscopes with digital imaging displays -- Information provided to the user regarding imaging performance , International Organization for Standardization (ISO) , Geneva, Switzerland , 2016 , www. iso.org/standard/61810.html .


[10] A Schué, Beware of “Empty” Magnifi cation , Science Lab, Leica Microsystems, Wetzlar, Germany, 2008, www.leica-microsystems.com/science-lab/ beware-of-empty-magnification .


References [1] C Hoyer, Chair Technical Committee , ISO 8039:2014: Microscopes -- Values, tolerances and symbols for magnifi - cation , International Organization for Standardization (ISO) , Geneva, Switzerland , 2014 , www.iso.org/standard/ 66260.html .


[2] C Hoyer, Chair Technical Committee , ISO 8578:2012 : Microscopes -- Marking of objectives and eyepieces , International Organization for Standardization (ISO) , Geneva, Switzerland , 2012 , www.iso.org/standard/50988. html .


[3] C Hoyer, Chair Technical Committee , ISO 9345-1:2012: Microscopes -- Imaging distances related to mechanical reference planes -- Part 1: Tube length 160 mm , International Organization for Standardization (ISO) , Geneva, Switzerland , 2012 , www.iso.org/standard/61065.html .


[4] C Hoyer, Chair Technical Committee , ISO 9345-2:2014: Microscopes -- Imaging distances related to mechanical reference planes -- Part 2: Infi nity corrected optical systems , International Organization for Standardization (ISO) , Geneva, Switzerland , 2014 , www.iso.org/standard/64986. html .


[5] C Hoyer, Chair Technical Committee , ISO 10934-1:2002: Optics and optical instruments -- Vocabulary for microscopy -- Part 1: Light microscopy , International Organization for Standardization (ISO) , Geneva, Switzerland , 2002 , www.iso.org/standard/35112.html .


30


[11] J DeRose and M Doppler, What Does 30,000:1 Magnifi cation Really Mean? Some Useful Guidelines for Understanding Magnifi cation in Today’s New Digital Microscope Era, Science Lab, Leica Microsystems, Wetzlar, Germany, 2015, www.leica-microsystems.com/science-lab/ what-does-300001-magnifi cation-really-mean.


[12] Computer display standard , Wikipedia The Free Encyclopedia, Wikipedia Foundation, San Francisco, 2015, en.wikipedia.org/wiki/Computer_display_standard .


[13] Display resolution , Wikipedia T e Free Encyclopedia, Wikipedia Foundation, San Francisco, 2015, en.wikipedia.org/wiki/Display_resolution.


[14] CE Shannon , Proceedings IEEE 86 ( 2 ) ( 1998 ) 447 – 57 , ieeexplore.ieee.org/document/659497 .


[15] H Nyquist , Proceedings IEEE 90 ( 2 ) (2002 ) 280 – 305 , ieeexplore.ieee.org/document/989875 .


[16] S Bradbury , Basic Measurement Techniques for Light Microscopy , Royal Microscopy Society Handbook #23, Oxford University Press, Oxford, UK, 1991, pp. 1–105.


[17] CG Healey and AP Sawant , ACM Trans Applied Perception 9 ( 4 ) ( 2012 ); 20 : 1 – 21 .


[18] S Sugawara et al ., SMPTE Motion Imag J 117 ( 3 ) ( 2008 ) 23 – 29 .


[19] T Kratzer, Chair Technical Committee , ISO 8596:2017: Ophthalmic optics -- Visual acuity testing -- Standard and clinical optotypes and their presentation , International


www.microscopy-today.com • 2018 July


Page 1  |  Page 2  |  Page 3  |  Page 4  |  Page 5  |  Page 6  |  Page 7  |  Page 8  |  Page 9  |  Page 10  |  Page 11  |  Page 12  |  Page 13  |  Page 14  |  Page 15  |  Page 16  |  Page 17  |  Page 18  |  Page 19  |  Page 20  |  Page 21  |  Page 22  |  Page 23  |  Page 24  |  Page 25  |  Page 26  |  Page 27  |  Page 28  |  Page 29  |  Page 30  |  Page 31  |  Page 32  |  Page 33  |  Page 34  |  Page 35  |  Page 36  |  Page 37  |  Page 38  |  Page 39  |  Page 40  |  Page 41  |  Page 42  |  Page 43  |  Page 44  |  Page 45  |  Page 46  |  Page 47  |  Page 48  |  Page 49  |  Page 50  |  Page 51  |  Page 52  |  Page 53  |  Page 54  |  Page 55  |  Page 56  |  Page 57  |  Page 58  |  Page 59  |  Page 60  |  Page 61  |  Page 62  |  Page 63  |  Page 64  |  Page 65  |  Page 66  |  Page 67  |  Page 68  |  Page 69  |  Page 70  |  Page 71  |  Page 72  |  Page 73  |  Page 74  |  Page 75  |  Page 76