Journal Highlights
Techniques
Automated Tools to Advance High-Resolution Imaging in Liquid by GM Jonaid, MA Casasanta, WJ Dearnaley, S Berry, L Kaylor, MJ Dressel-Dukes, MS Spilman, JL Gray, and DF Kelly, Microsc Microanal |
https://doi.org/10.1017/S1431927621013921. Liquid-electron microscopy (Liquid-EM) is an exciting
area in the materials imaging field providing unprecedented views of molecular processes. Time-resolved insights from Liquid-EM studies are a strong complement to the remarkable results achievable with other imaging techniques. Here, we describe opportunities to expand Liquid-EM technology by enhancing current practices with automated tools. Our results describe high-resolution structures of human viruses and individual proteins in liquid droplets by improving procedures for specimen preparation, data collection procedures, and computational processes. To develop these strategies, we used biological specimens relevant for drug delivery and the treatment of COVID-19. We also provide the first view of therapeutic protein candidates live in solution (Figure). Improving our understanding of the physical properties of macromolecules in a liquid state, as maintained in the human body, has broad societal implications for human health and disease. Major findings from this work entail insights for visualizing biological materials as well as quantifiable measures to assess their physical changes.
High-resolution structure of AAV determined from particles in solution. Slices through the AAV assembly with the atomic model (blue; pdb code, 3KIC, all chains) placed in the EM map (gray). A magnified region near the 5-fold axis shows some side chains present and distinct within the density. Scale bar = 5 nm.
A top journal in Microscopy
Published for the Microscopy Society of America Editor: John Mansfield, University of Michigan, USA
The only journal owned by scientists and published for scientists, Microscopy and Microanalysis provides original research papers in the fields of microscopy, imaging and compositional analysis. This distinguished international forum is intended for microscopists in both biology and materials science.
Online submission at
cambridge.org/mam/submit View the journal online at
cambridge.org/mam Microscopy Society of America 2022 May •
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