LED Illumination for Fluorescence
of wavelengths. Te system allows ratiometric imaging of Fura-2 at the low end of the spectrum, and coverage extends all the way to the near-IR for deeper imaging with Cy7, ICG, or IR800. High power, rich spectrum, and convenient design features make the X-Cite NOVEM more than just an arc lamp replacement. Te X-Cite NOVEM can be used in any laboratory or imaging instrument that requires fluorescence excitation. Te nine-channel switching capability allows for fast imaging for high-throughput imaging but also allows users in a multi- imaging facility to benefit from all the wavelengths. Whether the users are interested in Fura-2, or any of the popular fluores- cence proteins (BFP, CFP, GFP, YFP, RFP), one system can serve all these applications of interest. Te NIR version of NOVEM will be for users who want to image in the near-infrared fluo- rescence region. Interest for this region (using ICG and IR800) is growing because of its potential use in image-guided surgery.
References [1] CE Lyman, Microscopy Today 29 (2021) https://doi .org/ 10.1017/S1551929521001085.
[2] OL Barreto-Chang and RE Dolmetsch, J Vis Exp 23 (2009)
https://doi.org/10.3791/1067.
[3] WG Wier et al., Can J Physiol Pharmacol 66 (1988) https://
doi.org/10.1139/Y88-202.
[4] R Ma et al., Intl J Nanomedicine 16 (2021) https://doi .org/ 10.2147/IJN.S318678.
[5] CE Lyman, Microscopy Today 24 (2016) https://doi .org/ 10.1017/S1551929516000754.
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