Biological Applications
Structural Analysis of Gliding Motility of a Bacteroidetes Bacterium by Correlative Light and Scanning Electron Microscopy (CLSEM) by Devanshi Khare, Pallavi Chandwadkar, and Celin Acharya, Microsc Microanal |
https://doi.org/10.1017/ S1431927622000095.
We used correlative light and scanning electron microscopy
(CLSEM) to illustrate dynamic spatial and temporal analysis of microbial processes by light microscopy, and ultrastructural details with scanning electron microscopy. CLSEM provided complementary information from the same region of interest at any fixed point in time. Te members of the Bacteroidetes phylum move on the solid surfaces by gliding motility, leading to the formation of spreading colonies. We evaluated the structural features of the spreading colony edges in a uranium- tolerant Bacteroidetes bacterium, Chryseobacterium sp. strain PMSZPI, by CLSEM (Figure). We successfully acquired an optimal overlay/correlation of the light/fluorescence microscopy information of the cellular organization at the colony edges in the absence and presence of uranium. Te rod-shaped cells at the colony edges were perfectly packed in hexagonal clusters, aligning with the neighboring cells, and formed regular lattice patterns. Subsequently, imaging of the correlated regions was done at higher resolution in the scanning electron microscope to obtain more comprehensive information.
CLSEM of spreading colony edges of gliding Chryseobacterium PMSZPI in the presence of uranium. Overlay of LM and SEM images after correlation is shown (top). The yellow square (top) shows the area selected for higher-resolution imaging with SEM (bottom). The rod-shaped cells of PMSZPI appeared to be packed in regular lattices creating a “mesh”-like pattern.
Materials Applications
Disentangling Coexisting Structural Order Trough Phase Lock-In Analysis of Atomic-Resolution STEM Data by BH Goodge, I El Baggari, SS Hong, Z Wang, DG Schlom, HY Hwang, and LF Kourkoutis, Microsc Microanal |
https://doi.org/10.1017/ S1431927622000125.
Phase demodulation analysis of atomic-resolution S/TEM
images is a powerful way of visualizing crystalline lattice inhomogeneities such as defects and strain fields in real space. Many quantum materials, however, are described by complex and oſten coexisting structural order parameters, including not just the primary atomic lattice but also subtle structural distortions that form periodic superstructures. We demonstrate the extension of conventional geometric image phase analysis to secondary and superlattice frequency components beyond the primary lattice frequencies by a frequency lock-in method. Our technique is sensitive to phase slips and dislocations in superlattice order (for example, pm-scale antipolar displacements) even in regions of atomically pristine crystalline lattice (Figure). With the advantages of a Fourier-based technique, this method provides quantitative mapping of competing modes of atomic- scale superstructure order and disorder across mesoscale fields of view. Tis approach will enable direct visualization of the interplay between coexisting order parameters and provide new insights into the multiscale hierarchies of emergent phenomena in complex materials.
56 doi:10.1017/S1551929522000578
Phase lock-in mapping of the [1½0] superlattice peak describing antipolar displacements in a free-standing oxide membrane. Rich heterogeneity in the superlattice order can be observed across the 0.5 × 0.5 μm2
atomic-resolution
ADF-STEM image. The inset shows a region of pristine crystallinity with a dis- continuity of the antipolar displacements identified by a 휋 phase slip.
www.microscopy-today.com • 2022 May
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