Laser Scanning Multiphoton Microscopes
Figure 1: A) The MPX-1040 is an easy-to-use and portable multimodal imaging platform with an integrated turnkey femtosecond fiber laser that is optimized for biological imaging applications. The high flexibility and working distance of the system allows multiple experiments in an upright configuration (B,D) and can easily be modified to an inverted setup (C). B) Live mouse imaging of the cortex (Prof. Bruno Weber – University of Zurich). C) Inverted microscopy of a microfluidic system “CellLego” to image acoustofluidic generated 3D cavities for cell experiments (Bettina Sailer, Thomas Kellerer – TranslaTUM). D) Home office use during COVID-19 pandemic showing the MPX setup in the basement of Prof. Lamy’s home (Prof. Christophe Lamy - University of Geneva, Switzerland).
spectroscopy (CARS), stimulated Raman spectroscopy (SRS), and fluorescence lifetime imaging (FLIM) are all used in research. Tey all have unique advantages and disadvantages; however, combining modalities maximizes informational con- tent and yields complementary data [4,5]. Also, merging tra- ditional (widefield/brightfield) epi-fluorescence and lifetime imaging techniques adds even more imaging power (Figure 2). SHG and two-photon imaging are powerful diagnostics
when used in unison for differentiating muscles, tendons, and cartilage (collagens) from surrounding tissues, providing endogenous molecular and chemical distinction [5]. Label-free contrast from colors enhances image specificity necessary for
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understanding the relationship between healthy, damaged, or pathological tissue and to engineer replacement materials tailored to the specific needs of the patient. Te MPX allows the user to electronically toggle between imaging modes for dynamic control of excitation and detection along one opti- cal path. As changing devices is not required, this saves time and effort and preserves placement and focus on the region of interest. Te MPX can investigate a large variety of sample types,
including formalin-fixed-paraffin-embedded (FFPE)
samples, cryosections, 3D tissues, and whole animals, which is a premium necessity for scientific researchers. Figure 3 gives a few examples demonstrating these benefits and the advantages
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