Carmichael’s Concise Review Coming Events
Due to COVID-19, please check to see if the listed events have been postponed or cancelled.
2020
16th International Congress of Histochemistry and Cytochemistry (ICHC)
August 30–September 2, 2020 Prague, Czech Republic
http://ichc2020.com
Neuroscience 2020 October 24–28, 2020
Washington, DC
www.sfn.org/meetings/neuroscience-2020
2020 MRS Fall Meeting & Exhibit
November 29–December 4, 2020 Boston, MA
www.mrs.org/fall2020
ASCB 2020 Annual Meeting
December 5–9, 2020 Philadelphia, PA
www.ascb.org/meetings-events/future-ascb-meetings
2021
Microscopy & Microanalysis 2021 August 1–5, 2021
Pittsburgh, PA
www.microscopy.org
2022
Microscopy & Microanalysis 2022 July 31–August 4, 2022
Portland, OR
www.microscopy.org
2023
Microscopy & Microanalysis 2023 July 24–28, 2023
Minneapolis, MN
www.microscopy.org
2024
Microscopy & Microanalysis 2024 July 28–August 1, 2024
Cleveland, OH
www.microscopy.org
Opening a New Window for Observing Embryogenesis
Stephen W. Carmichael Mayo Clinic, Rochester, MN 55905
carmichael.stephen@
mayo.edu
Embryonic development is a complex process that is difficult to observe directly.
Histologic examination of fixed embryos does not capture the dynamics of devel- opment. Other imaging methods have limitations, such as low resolution and the inability to take advantage of transgenic strains with fluorescent reporters. Studies with superresolution microscopy of specimens in vitro has limitations, such as the inability to mimic the uterine environment past embryonic day 9 (E9) when various organs form. A recent study [1] by a large international group led by Qiang Huang, Rudolf Jae-
nisch, and Xiling Shen demonstrated that it is possible to overcome some of these limitations by implanting a window into the uterus of pregnant mice (dams). Tis allows intravital imaging that can be used to observe the formation of organs at high resolution from E9.5 to birth. Te embryo becomes easily identifiable under a dissec- tion microscope at about E9.5 when the allantois fuses with the chorionic plate result- ing in a large surface area for gas and nutrient exchange. Initially an optical window (and a clip to stabilize the window) was designed
by Huang et al. and fabricated by 3D printing. A round incision was made in the abdominal wall, and some skin was removed. Te uterus was exteriorized, and an embryo near an ovary was selected. Te window was implanted in the uterine wall, the embryo was sutured to the abdominal muscle, the abdominal muscle was sutured to
Figure 1: The image in the upper left corner is of an embryo at E15.5. The color images show diffusion of fluorescein into an embryo after it was injected into the dam. The upper left color image was taken 1.5 minutes after the injection,
the upper right image taken at 15 minutes, 30 minutes, and lower right at 15 minutes. Scale bar = 2 mm. 8 doi:10.1017/S1551929520001297 2020 September lower left image taken at
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