2020 Microscopy Today Micrograph Awards


Open 2nd


Prize. This discharged nematocyst of a Portuguese Man O’War (Physalia physalis) was collected from the Atlantic Ocean off the coast of Florida. The


spherical nematocyst capsule (blue), ejected from its nematocyte, is still covered by a microtubular basket (orange). SEM secondary electron image acquired at 4 kV. Image by Connon Thomas, Max Planck Florida Institute for Neuroscience, Jupiter, FL. Open 3rd


Prize. Rat endothelial cells fixed on a glass slide and marked with fluorescent dyes. Nuclei were stained with DAPI (blue), actin filaments with phalloidin


(green), and mitochondria with MitoTracker™. Sample from Anja Kraemer of Boehringer Ingelheim Pharma GmbH. Image acquired with a confocal Raman micro- scope. Image by Damon Strom, WITec GmbH, Ulm, Germany.


microscopists to think about image composition and visual impact in experiment planning and during image acquisition. Tird, the understanding of mechanisms and processes oſten requires dynamic imaging acquired by in situ microscopy of all types. Tus, we have established a separate category for video micrographs. Tis category also includes digital animations of reconstructed three-dimensional datasets, for example from cryo-electron microscopy, that provide new insights into cellu- lar and even molecular structures.


Video Category Our competition is also driven by image quality from a


technical standpoint. Sharpness of image details is important. Imaging of three-dimensional objects with a large depth of field was once the exclusive domain of the scanning electron micro- scope, but with focus-stacking soſtware,


light micrographs


now can be in sharp focus over a considerable depth of field. Our judges evaluated submitted micrographs on large high- resolution monitors that can reveal lack of sharpness, as well as other image defects. We request that submitted images have


Video 1st


Prize. Soap film colors resulting from interference of reflected light beams: some incident light reflects off the top surface while the remaining light travels


to the back of the film and is reflected. Color variations are produced as the thickness of the film varies across the field. Bright-field light microscopy. Image by Gerd Günther, independent microscopist, Düsseldorf, Germany.


16 www.microscopy-today.com • 2020 September


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