Neural Stem Cells
Acknowledgments We are grateful to the ZEISS team
Figure 6: Dr. Espinosa-Jeffrey imaging with ZEISS Axio Observer 7.
members who supported the live micros- copy needs for the post-flight part of this project: Mark Mobilia – Account Man- ager for UCLA; Dr. Neeraj Gohad – Life Sciences PASS Manager, West; Dr. Ben Ng – Field Applications Scientist; Kevinne Aguilar – Local Service Engineer; Stefan Gross – Regional Sales Manager West, US; Tanya Mahboob – Demonstration Inven- tory Coordinator. Without their invaluable support, this part of our study would not have been possible. With the ZEISS time- lapse system we were able to capture unique properties displayed by human neural cells aſter space flight. Tanks to Dr. Amy Gresser. Elizabeth Pane, and Medaya Tor- res for their help with the implementation of the study; Te NASA Space Biology Project team at Ames Research Center including Dr. David Tomko, Dr. Kevin Sato, Elizabeth Taylor, ARC Space Biology Project Manager, and the support person- nel at the Space Station Processing Facility at Kennedy Space Center; to Karin Perkins, Diana Ly, and Tino Hernandez for their invaluable support; to Maria Birlem and Chriss Bruderrek from Yuri, and STaARS team members Tom Kyler, Craig Walton, and BreAnne MacKenzie for supporting the flight implementation; to Uli Kue- bler from Airbus Defense and Space, who introduced us to the flying hardware used for this study; and to the IDDRC Cell Cul- ture Core.
Figure 7: Proliferation of neural stem cells cultured post-exposure to zero gravity showing cell division (asterisk and arrowhead).
that neuronal stem cells that have flown in space become neu- rons when cultured in the appropriate conditions. Tey have also used the ZEISS LSM 800 confocal microscope to confirm their neuronal nature [6].
Conclusion Te information reported here is very much a work in prog-
ress, and the proliferation of neural stem cells in space must be studied in more detail to determine the possible relationship to the visual impairment and intracranial pressure syndrome exhibited by astronauts in space and upon their return to Earth. Learning more is essential to be able to design effective counter- measures for astronauts working onboard the ISS and for long- term space travel. Te next phase of the work will be performed on SpaceX-21, a commercial resupply service mission to the ISS using the new Dragon 2 spacecraſt, planned for October 2020.
2020 September •
www.microscopy-today.com
Funding We thank NASA Space Biology
for Grant NNX15AB43G; the IDDRC Cell Culture Core is supported by NIH/ NICHD grant number U54HD087101-05.
References [1] A Espinosa-Jeffrey et al., PLoS One 8 (2013) e76963 1–10. [2] A Espinosa-Jeffrey et al., J Neurosci Res 94 (2016) 1434–50. [3] J Green et al., “Te Implications of Microgravity on Cell Morphology and Proliferation of Stem Cell Progenies Towards Astronaut Health,” International Astronautical Federation (IAF), IAC-16-A1,7,9,x35726 (2016), Guadala- jara, Mexico.
http://iafastro.directory/iac/paper/id/35726/ summary.
[4] A Espinosa-Jeffrey et al., Applied Sciences (2020) (in press). [5] A Espinosa-Jeffrey et al., J Neurosci Res 69 (2002) 810–25. [6] C Cepeda, Appl Sci 9 (2019) 4042. [7] NASA, About Bioscience-04 (SpaceX-16). https://www.
nasa.gov/ames/research/space-biosciences/bioscience- 4-spacex-16.
29
Page 1 |
Page 2 |
Page 3 |
Page 4 |
Page 5 |
Page 6 |
Page 7 |
Page 8 |
Page 9 |
Page 10 |
Page 11 |
Page 12 |
Page 13 |
Page 14 |
Page 15 |
Page 16 |
Page 17 |
Page 18 |
Page 19 |
Page 20 |
Page 21 |
Page 22 |
Page 23 |
Page 24 |
Page 25 |
Page 26 |
Page 27 |
Page 28 |
Page 29 |
Page 30 |
Page 31 |
Page 32 |
Page 33 |
Page 34 |
Page 35 |
Page 36 |
Page 37 |
Page 38 |
Page 39 |
Page 40 |
Page 41 |
Page 42 |
Page 43 |
Page 44 |
Page 45 |
Page 46 |
Page 47 |
Page 48 |
Page 49 |
Page 50 |
Page 51 |
Page 52 |
Page 53 |
Page 54 |
Page 55 |
Page 56 |
Page 57 |
Page 58 |
Page 59 |
Page 60 |
Page 61 |
Page 62 |
Page 63 |
Page 64 |
Page 65 |
Page 66 |
Page 67 |
Page 68 |
Page 69 |
Page 70 |
Page 71 |
Page 72 |
Page 73 |
Page 74 |
Page 75 |
Page 76 |
Page 77 |
Page 78 |
Page 79 |
Page 80
