Biological Applications
A Simple and Rapid Staining Technique for Sex Determination of Trichinella Larvae Parasites by Confocal Laser Scanning Microscopy by I Gavarane, E Kirilova, I Rubeniņa, L Mežaraupe, S Osipovs, G Deksne, A Pučkins, I Kokina, A Bulanovs, and M Kirjušina, Microsc Microanl | doi:10.1017/S1431927619015046
A novel fluorescent benzanthrone dye 3-N-[2-(4-
morpholinyl)acetamido] has been developed for simple and rapid staining of parasitic roundworms such as Trichinella, a parasite of carnivorous and omnivorous animals. Trichinella can be transmitted to humans by consumption of uncooked infected meat products result- ing in Trichinellosis, which can be fatal depending on the infective dose. Current staining protocols for Trichinella are time-consuming and labor-intensive. Using fixed T. spiralis and T. britovi larvae, the new preparation method takes approximately 5 minutes. Use of confocal micros- copy and the new benzanthrone fluorescent dye makes examination of internal and external structures possible, and the sex of larval samples can be determined by mea- surement of the rectal length (Figure). Results confirmed that the larval male rectum is approximately two times larger than the female rectal length.
Material Applications
Hollow Silver Nanostructures: Te Role of Capping Agents in Tailoring the Shape, Structure, and Plasmonic Properties by BK Dadhich, B Bhushan, and A Priyam, Microsc Microanl | doi:10.1017/S1431927619000473 Due to single, intense, and highly tuneable plasmon
peaks, hollow metal nanostructures have caught the imagination of scientists worldwide. Nanoshells of noble metals, particularly those of gold and silver, have a wide range of applications including catalysis and theranostics. In this work, the shape- and structure-directing ability of folic acid (FA) and acetic acid (AA) capping agents were investigated. FA transformed both the shape and struc- ture of solid spherical Ag2
O nanoparticles (NPs) resulting
in hollow nanocubes (HAgNCs). In contrast, acetic acid (AA) acted only as a structure-directing agent resulting in the transformation of solid Ag2
O nanospheres into hol-
low nanospheres (HAgNSs) (Figure). FA-capping leads to enhanced plasmon tunability ranging from 535–640 nm in the hollow Ag2
O NPs. High-resolution transmis-
sion electron microscopy revealed that the outer diameter of AA-capped HAgNSs was 50±10 nm, while the edge- length for FA-capped HAgNCs was 100±15 nm. Te diameter of the inner void space was 30±5 nm and 43±5 nm for the HAgNSs and HAgNCs, respectively.
58 doi:10.1017/S1551929519001378 Spherical Ag2 O nanoparticles were transformed into hollow spheres (left) or
cubes (right) by AA or FA. The transformation from spherical to cubical is attrib- uted to the shape- and structure-directing ability of FA.
www.microscopy-today.com • 2020 March
Confocal image of Trichinella spiralis female larva indicating the region of the rectum (<->).
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