Volume 28 Number 2 2020 March


General Interest


12 Order! Order! John C. Russ


Contents Single-Molecule Localization


20 Fast Analysis of 2D and 3D Single-Molecule Localization Microscopy Data with Huygens Localizer Peter J. Verveer, Daniel Sevilla-Sánchez, Paul C. Bloembergen, Nancy Adam, Vincent T.G. Schoonderwoert, and Hans T.M. van der Voort


Spectroscopy


26 Technological Advances Make Low-Cost Infrared Microspectroscopy Routine Bethany Renstrom, Kelly Minuskin, Bryce Egan, André J. Sommer, David Schiering, and Gregg Ressler


34 Effect of the Silicon Drift Detector on EDAX Standardless Quant Methods Frank Eggert


Microscopy 101 About the Cover


40 Recipes for Consistent Selected Area Electron Diffraction Results: Part 1: Microscope Setup Scott D. Walck


Microscopy Education


46 An Interactive Online Electron Microscopy Platform Integrating Classroom Lectures and Lab Practice Noah Freed, Devon Butler, Allison Cairns, Rick Hugo, Jun Jiao, and Zhiqiang Chen


Highlights from Microscopy and Microanalysis


58 A Simple and Rapid Staining Technique for Sex Determination of Trichinella Larvae Parasites by Confocal Laser Scanning Microscopy


Super-resolution single molecule localization microscopy (SMLM) analysis using Huygens Localizer software. For details please see the article by Verveer et al.


58 Hollow Silver Nanostructures: The Role of Capping Agents in Tailoring the Shape, Structure, and Plasmonic Properties


59 HAADF-STEM Image Resolution Enhancement Using High-Quality Image Reconstruction Techniques: Case of Fe3


O4 Departments


7 Editorial 8 Carmichael’s Concise Review


52 Pioneers 54 Industry News 56 Product News


60 NetNotes 68 Calendar of Meetings 73 Dear Abbe 74 Index of Advertisers


(111) Surface


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