Webinar: Quantification of proteins in complex biological samples by LC–MS/MS


In this mini-webinar, Rainer Bischoff (University of Groningen, Te Netherlands) investigates the different LC–MS/MS approaches for quantitative protein bioanalysis with focus on biopharmaceuticals, biomarkers and targeted proteomics. Until recently, the only way to quantify proteins in complex biological matrices like plasma or serum was by ligand binding assays using antibodies or antibody-like affinity ligands, usually in the ELISA format. Recent developments in LC–MS/MS have opened the possibility to quantify proteins by selected reaction monitoring at low concentration levels making the technique a viable alternative to ligand binding assays. Te use of triple quadrupole mass analyzers is discussed -the workhorse instrument for the quantitative bioanalysis of proteins.


What will you learn? • How to set up a targeted LC–MS/MS method for a given protein • How different approaches to sample preparation and internal standards affect sensitivity • How LC–MS/MS assays can be multiplexed


Watch the full webinar on demand at: www.bioanalysis-zone.com/2017/04/18/22246_waters/


www.bioanalysis-zone.com


51


Page 1  |  Page 2  |  Page 3  |  Page 4  |  Page 5  |  Page 6  |  Page 7  |  Page 8  |  Page 9  |  Page 10  |  Page 11  |  Page 12  |  Page 13  |  Page 14  |  Page 15  |  Page 16  |  Page 17  |  Page 18  |  Page 19  |  Page 20  |  Page 21  |  Page 22  |  Page 23  |  Page 24  |  Page 25  |  Page 26  |  Page 27  |  Page 28  |  Page 29  |  Page 30  |  Page 31  |  Page 32  |  Page 33  |  Page 34  |  Page 35  |  Page 36  |  Page 37  |  Page 38  |  Page 39  |  Page 40  |  Page 41  |  Page 42  |  Page 43  |  Page 44  |  Page 45  |  Page 46  |  Page 47  |  Page 48  |  Page 49  |  Page 50  |  Page 51  |  Page 52  |  Page 53  |  Page 54  |  Page 55  |  Page 56  |  Page 57  |  Page 58