The Collectables: Year in Bioanalysis


ionization suppression or enhancement. Interferences from endogenous proteins may affect accuracy, repro- ducibility and method ruggedness. Method sensitiv- ity is also strongly impacted by sample complexity. The use of selective immunocapture techniques to remove interfering matrix proteins before diges- tion will reduce sample complexity and minimize interferences. Peptide level cleanup after digestion


may be also required in some cases. Q


What further advances do you envisage with LC–MS/MS in the future?


I anticipate more high resolution MS applications for biotherapeutics and on the chromatographic separa- tion side, I expect more technological discoveries in protein/peptide separation.


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