BIOANALYSIS ARTICLE ABSTRACTS
and conventional sampling methods with commercial and in-house molecules. The plasma concentrations of the molecules tested were very consistent between the conventional sampling techniques and this new device/workflow. In addition, several in-life studies have also been conducted to validate this new technique as a primary PK screening tool at the Takeda Boston. The new device is simple to use and very cost effective with the added benefit that no additional training is needed for the animal technicians and the same centrifuge equipment can be employed. This device can be used for blood volumes ranging from 20 to 100 µl enabling studies not just in rat and dog but more importantly in mice.
8(9), 871–880;
www.future-science.com/doi/full/10.4155/bio.16.26
Comparison of blood microsampling with DBS and conventional blood collection techniques used in a midazolam biostudy
46
Background: Quantitative DBS LC–MS/MS assay for midazolam was used to compare two sample collection techniques (venipuncture and finger prick) and the midazolam concentrations measured in plasma samples, DBS and dried plasma spots. Methodology: Midazolam was extracted from DBS cards and compared with whole blood collected from usual venipuncture. Dried plasma spots were also compared with plasma. The blood volume used as well as the temperature impact during the blood and plasma deposits was evaluated. Midazolam was administrated to six healthy subjects during a clinical trial to obtained blood and plasma samples for the statistical comparison. Conclusion: The method for midazolam using DBS was validated and showed an excellent performance. Excellent correlations were observed when the same collection procedures were used.
8(8), 741–751;
www.future-science.com/doi/full/10.4155/bio-2015-0031
www.bioanalysis-zone.com
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