BIOANALYSIS ARTICLE ABSTRACTS
BIOANALYSIS ARTICLE ABSTRACTS
Effect of time on recovery of plasma microsamples for the quantitative determination of vancomycin
The reliability of extraction recovery of an analyte in bioanalysis is fundamentally important for downstream analytical testing. For dried format microsamples, if the recovery changes with time the concentration in clinical samples, derived from calibration standards and alongside quality control samples prepared following different drying protocols, may not reflect the true result. The purpose of this paper was therefore to evaluate changes to extraction recovery across time for one analyte, the glycopeptide antibiotic vancomycin, in plasma using two dried microsampling formats, dried plasma spots and volumetric absorptive microsampling.
8(21), 2235–2242;
www.future-science.com/doi/full/10.4155/bio-2016-0159
Automated DBS microsampling, microscale automation and microflow LC–MS for therapeutic protein PK
Aim: Reduce animal usage for discovery-stage PK studies for biologics programs using microsampling-based approaches and microscale LC–MS. Methods & results: We report the development of an automated DBS-based serial microsampling approach for studying the PK of therapeutic proteins in mice. Automated sample preparation and microflow LC–MS were used to enable assay miniaturization and improve overall assay throughput. Serial sampling of mice was possible over the full 21-day study period with the first six time points over 24 h being collected using automated DBS sample collection. Overall, this approach demonstrated comparable data to a previous study using single mice per time point liquid samples while reducing animal and compound requirements by 14-fold. Conclusion: Reduction in animals and drug material is enabled by the use of automated serial DBS microsampling for mice studies in discovery-stage studies of protein therapeutics.
8(7), 649–659;
www.future-science.com/doi/full/10.4155/bio-2015-0006
Development of a novel noncapillary plasma microsampling device for ultra-low volume of blood collection
The desire for serial microsampling in mice has led to extensive research in this field within the pharmaceutical industry. The ability to profile a compound’s in vivo properties with less material and fewer mice has obvious advantages. A new device and workflow was developed at the Takeda Oncology site to allow scientists to isolate plasma from very low volumes of mouse blood (as low as 20 µl) collected using standard microsampling techniques. A side- by-side in vitro comparison of plasma concentrations was performed using this new device
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