Microscopy & Microtechniques 79 Cryo-SEM Preparation System Helps Researchers Conduct Research into Plant Biology
Quorum Technologies report on plant biology research at the Sainsbury Laboratory, Cambridge UK, where their PP3010T Cryo-SEM preparation system is in routine use.
The Sainsbury Laboratory Cambridge University (SLCU) is a new research institute funded by the Gatsby Foundation. The Laboratory hosts a state-of-the-art advanced imaging facility for scientists working on several aspects of plant developmental biology, including live imaging of developing plant tissues, and high-resolution scanning electron microscopy. The facility currently has four major instruments, two stereo-fluorescence microscopes and several dissecting microscopes. Dr Raymond Wightman is its Microscopy Core Facility Manager.
Studying plants using scanning electron microscopy (SEM) was a challenge. Prior to having a Cryo-SEM preparation system, the lab would look at fresh plant samples that dried up and were hard to maintain while doing SEM. There was an urgent requirement for something to enable better imaging of fully hydrated plant tissue.
Asked about his experience using the Quorum PP3010T Cryo-SEM preparation system on the Zeiss EVO HD cryoSEM, Dr Wightman said: “We have been impressed with the low maintenance costs and excellent applications support from the Quorum team. The system is now an integral part of the microscopy facility at the Sainsbury lab and because of the superior results we are getting now compared to before, the SEM use has gone up from about 8 hours per month to 64 hours per month. We are also able to process samples that we could not easily do prior to getting the Quorum kit.”
The system has been used in multiple projects by the Laboratory. These have included looking at the following: wood ultrastructure and cellulose organisation; observing cellular membranes; making new biocomposites and studying their properties; studying the interactions between a plant pathogen and the plant cell; studying cuticular wax formation; determining how plant cells coordinate their growth across tissues e.g. leaves and flowers. A final project on the development and structure of leaves from alpine plants where 90% of the work was performed on the PP3010T will be published shortly. All of these serve to illustrate the versatility of the Quorum cryo-SEM preparation system.
The PP3010T is a highly automated, easy-to-use, column-mounted, gas-cooled cryo-SEM preparation system suitable for most makes and models of SEM, FE-SEM and FIB/SEM.
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Fast Acquisition Mode for LSM 880 with Airyscan Introduced
Zeiss is expanding the capabilities of the Airyscan detector for confocal laser scanning microscopes (LSM) to applications demanding the highest imaging speeds. The Fast module for Zeiss LSM 880 with Airyscan enables parallel excitation and detection of four image pixels. The result is a speed improvement by a factor of four, while maintaining the outstanding sensitivity of Airyscan and 1.5x resolution improvement. The gain in imaging speed allows researchers to enter the domain of classic resonant scanning systems, but with a much better signal-to-noise ratio. Zeiss LSM 880 with Airyscan can be used for single and multiphoton experiments, providing full flexibility for life science applications.
The Fast module for ZEISS LSM 880 with Airyscan complements the leading platform for confocal live cell imaging. Starting today, researchers can
experience hands-on experiments with their own samples in selected worldwide ZEISS demo centres. Existing ZEISS LSM 880 systems can easily be upgraded on site with the Fast module.
Released in 2014, the Airyscan detector quickly established itself as a new standard in confocal live cell imaging. Since then, scientists have already used the increased resolution in all spatial dimensions and the highest sensitivity of Airyscan to publish exciting new data in high-ranking scientific journals. The Zeiss LSM 8 family with Airyscan collected multiple prestigious awards, including an R&D 100 Special Recognition Award, a Scientist’s Choice Award for best new life science product, and the Innovation Prize of the State of Thuringia.
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