Chromatography 23


Manufacturer-Packed HPLC Columns for Every Application MACHEREY-NAGEL, a pioneer in chromatography, introduced its NUCLEOSIL®


NUCLEOSIL® silica was originally developed by them in Germany. The manufacturer-packed NUCLEOSIL® columns packed with a superficially porous silica for highest efficiency in HPLC. silica provide phases for many individual applications. , C6 H5 ,CN, NH2


HPLC columns into the market more than 40 years ago. The spherical columns are now exclusively offered by


MACHEREY-NAGEL and authorised distributors. For nearly 15 years, they have supplemented their successful NUCLEOSIL® packed with the fully synthetical silica NUCLEODUR® NUCLEOSHELL®


NUCLEOSIL®


because the silica is robust. Numerous different modifications (e.g., C18 120, 300, 500, 1000, 4000 Å) of NUCLEOSIL®


column range by columns – a state-of-the-art silica for professional solutions. A few years ago, MACHEREY-NAGEL introduced


columns – until now also packed by other column manufacturers – are often used in quality control around the globe for validated methods , C8


Further information can be found on www.mn-net.com/NUCLEOSIL For daily routine analysis as well as for sophisticated analytical separations, NUCLEODUR®


for separations of basic and acidic analytes with high peak symmetry. They are also ideal for method development, LC-MS detection and UHPLC. Further selectivities are provided with CN/CN-RP and NH2


are recommended. Several different surface modifications have been developed over the last years providing a full range of specified HPLC phases. Surface deactivated phases like C8


and C18 for low molecular analytes – C18 ec and C4 ec with 300 Å for proteins and peptides. NUCLEODUR®


Gravity, C18 /NH2


Gravity-SB, C18 -RP phases. C18


ec and C8 Isis, C18


More information on www.mn-net.com/NUCLEODUR Core-shell technology sets new standards for analyses in research and quality control. NUCLEOSHELL®


C18 More information on www.mn-net.com/NUCLEOSHELL


Decades of experience in phase development, sorbent manufacturing and column packing by MACHEREY-NAGEL obtain safe and reliable results in HPLC. A wide variety of applications are provided in their free-of-charge application database www.mn-net.com/apps


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Pyramid, PolarTec, Phenyl-Hexyl, Sphinx RP, PFP and HILIC are well-suitable ec modifications with a pore size of 110 Å are modern standard phases


HTec is the preferred phase for preparative HPLC. columns fulfill the demands for HPLC separations


with respect to separation efficiency, detection limits, and time requirements for each analysis. MACHEREY-NAGEL offers the core-shell phases RP 18, RP 18plus, Phenyl-Hexyl, PFP, and HILIC with the highly efficient particle size of 2.7 µm. Especially for an allowed transfer of Pharmacopoeia methods, the important modifications NUCLEOSHELL®


RP 18 and more polar RP 18plus are also available with the particle size of 5 µm. columns manufacturer-packed with high pressure stable silica , SA, SB), particle sizes (3, 5, 7, 10 µm) and pore sizes (50, 100,


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