14
New Released N-Glycan Assay Introduced
Sartorius Stedim Biotech (SSB), a leading international supplier for the biopharmaceutical industry, have announced the launched of a new Released N-Glycan Assay for the in-depth profiling of IgG glycosylation. This new physico-chemical assay, developed by SSB’s subsidiary BioOutsource and offered in combination with its unique Antibody Dependent Cell Cytotoxicity (ADCC) assay platform, combines data on the molecular structure with a key therapeutic mechanism of action. This will generate comprehensive, highly accurate biosimilar comparability data, driving shorter drug development timelines.
6750ad@reply-direct.com
Benchtop Spectrometer Helps to Teach Students the Practical use of NMR for Spectroscopic Characterisation in Organic Chemistry
Magritek reports on the benefits of their Spinsolve Benchtop NMR spectrometer for teaching practical organic chemistry to undergraduate students at Cleveland State University in Ohio.
Dr Vania De Paoli is an associate college lecturer in the Department of Chemistry at Cleveland State University in Ohio. She is leading a program to create a solid environment for teaching Organic Chemistry. This focuses on critical thinking and analysis where every experiment is seen as a small project. The goal allows students to experience every experiment performed in lab as a (junior) scientist. Not only are synthetic skills important, but the students also need to be able to analyse their reagents and products to answer questions such as ‘does what I have made need extra purification’ or ‘what was my yield?’
Prior to investing in the Magritek benchtop NMR spectrometer, students’ practical options were limited to the measurement of melting points and refractive index. Old gas chromatographs were so old, they were no longer functional. Students were not experiencing anything close to life in a modern organic chemistry laboratory.
As Dr De Paoli says, the Spinsolve has changed this position immensely. “It is a small system, portable and lightweight. It is quickly ready to use allowing the students to have a real NMR analysis experience (they prepare the samples in standard NMR tubes, add the solvent and record the spectra in similar ways to a research grade NMR). The spectra are of good quality. The software is friendly and, overall, Spinsolve is readily affordable.”
To complement the spectrometer, Magritek provides a series of support aids to those teaching NMR. These include videos, practical experiment protocols and lab manuals. Supplied free of charge, these useful tools are available by clicking here:
http://www.magritek.com/applications/ chemistry-education/
39524pr@reply-direct.com
The new N-Glycan Assay is one of a range of orthogonal methods, including analysis by CD16a (FcγRIIIa) and SPR binding, as well as ADCC functional assays that BioOutsource offers to provide a detailed testing package to generate evidence suitable for regulatory submissions.
BioOutsource’s new assay involves enzymatically-removing glycans from the antibody, functionally derivatising them and separating the glycans by UHPLC. The glycans are then identified by on-line electrospray ionisation mass spectrometry (ESI-MS), enabling confident assignment and precise quantification of the different structures.
Dr Daniel Galbraith, Chief Scientific Officer at Sartorius Stedim BioOutsource, explained: “The structural classification and monosaccharide composition of carbohydrates attached to the constant region of therapeutic biosimilar antibodies is key to their binding. An accurate understanding of these glycans ensures effective selection of biosimilars that have the optimum ADCC activity to destroy tumour cells.”
Galbraith continued: “Our new Released N-Glycan Assay coupled with our ADCC assays will help deliver this understanding by providing robust biosimilar characterisation data suitable for the regulators, thus enabling efficient drug development and more rapid entry into clinical programs.”
To find out about BioOutsource’s new Released N-Glycan Assay and complementary
ADCC assays, scientists should go to
www.biooutsource.com/glycan-analysis/.
39593pr@reply-direct.com
Page 1 |
Page 2 |
Page 3 |
Page 4 |
Page 5 |
Page 6 |
Page 7 |
Page 8 |
Page 9 |
Page 10 |
Page 11 |
Page 12 |
Page 13 |
Page 14 |
Page 15 |
Page 16 |
Page 17 |
Page 18 |
Page 19 |
Page 20 |
Page 21 |
Page 22 |
Page 23 |
Page 24 |
Page 25 |
Page 26 |
Page 27 |
Page 28 |
Page 29 |
Page 30 |
Page 31 |
Page 32 |
Page 33 |
Page 34 |
Page 35 |
Page 36 |
Page 37 |
Page 38 |
Page 39 |
Page 40 |
Page 41 |
Page 42 |
Page 43 |
Page 44 |
Page 45 |
Page 46 |
Page 47 |
Page 48 |
Page 49 |
Page 50 |
Page 51 |
Page 52 |
Page 53 |
Page 54 |
Page 55 |
Page 56 |
Page 57 |
Page 58 |
Page 59 |
Page 60 |
Page 61 |
Page 62 |
Page 63 |
Page 64 |
Page 65 |
Page 66 |
Page 67 |
Page 68 |
Page 69 |
Page 70 |
Page 71 |
Page 72 |
Page 73 |
Page 74 |
Page 75 |
Page 76 |
Page 77 |
Page 78 |
Page 79 |
Page 80 |
Page 81 |
Page 82 |
Page 83 |
Page 84 |
Page 85 |
Page 86 |
Page 87 |
Page 88 |
Page 89 |
Page 90 |
Page 91 |
Page 92 |
Page 93 |
Page 94 |
Page 95 |
Page 96 |
Page 97 |
Page 98 |
Page 99 |
Page 100 |
Page 101 |
Page 102 |
Page 103 |
Page 104 |
Page 105 |
Page 106 |
Page 107 |
Page 108 |
Page 109 |
Page 110 |
Page 111 |
Page 112 |
Page 113 |
Page 114 |
Page 115 |
Page 116 |
Page 117 |
Page 118 |
Page 119 |
Page 120 |
Page 121 |
Page 122 |
Page 123 |
Page 124 |
Page 125 |
Page 126 |
Page 127 |
Page 128 |
Page 129 |
Page 130 |
Page 131 |
Page 132
