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SOS particles were deemed to be a suitable stationary phase to embark upon the new challenges in the field of biomolecules. The Initial evaluation of this material (without optimisation) have shown equivalent or better performance in separation of certain mixtures, compared to the conventional core–shell and wide pore silica particles. This prototype material was successfully applied for the analysis of model proteins and for therapeutic monoclonal antibody (mAb) and antibody-drug conjugate (ADC) samples. The kinetic performance of the SOS material was also evaluated and compared to that of other wide pore state-of-the-art fully porous and core–shell materials commonly employed to separate proteins. As illustrated, the performance achieved with the SOS column was comparable and sometimes better than other columns dedicated to proteins analysis and packed with particles ranging between 1.7 and 5 µm. The results were very promising for a prototype material and this clearly demonstrates there is a still lot of opportunity for optimisation.
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